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Appl. Environ. Microbiol., Nov 1997, 4600-4603, Vol 63, No. 11
Copyright © 1997, American Society for Microbiology

Combined Light Microscopy and Attenuated Total Reflection Fourier Transform Infrared Spectroscopy for Integration of Biofilm Structure, Distribution, and Chemistry at Solid-Liquid Interfaces

PA Suci, KJ Siedlecki, RJ Palmer Jr, DC White and GG Geesey
Center for Biofilm Engineering and Department of Microbiology, Montana State University, Bozeman, Montana 59717; Intel Corporation, Rio Rancho, New Mexico 87124; Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee 37831; and Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830

Reflected differential interference contrast microscopy and attenuated total reflection Fourier transform infrared spectroscopy were used to obtain complementary data on the structural and chemical properties of a biofilm. This information was obtained nondestructively, quasisimultaneously, and in real time, thereby permitting the verification of time-dependent relationships between the biofilm's population structure, distribution, and interfacial chemistry. The approach offers opportunities to examine these relationships on a variety of substrata in the presence of a bulk aqueous phase under controlled hydrodynamic conditions.


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