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Appl. Environ. Microbiol., Nov 1997, 4608-4611, Vol 63, No. 11
Copyright © 1997, American Society for Microbiology

Use of nucleic acid dyes SYTO-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry

T Guindulain, J Comas and J Vives-Rego
Departament de Microbiologia, Universitat de Barcelona, Spain.

Three nucleic acid dyes (SYTO-13, TOTO-1, and YOYO-1) were tested on cultures of Escherichia coli and marine prokaryote populations. These dyes stain the RNA and DNA in E. coli but only respond to DNA in marine populations, according to the histograms obtained after DNase and RNase treatments.


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  • Lebaron, P., Catala, P., Parthuisot, N. (1998). Effectiveness of SYTOX Green Stain for Bacterial Viability Assessment. Appl. Environ. Microbiol. 64: 2697-2700 [Abstract] [Full Text]  


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