This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Suarez, A. M.
Right arrow Articles by Hernandez, P. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suarez, A. M.
Right arrow Articles by Hernandez, P. E.
Agricola
Right arrow Articles by Suarez, A. M.
Right arrow Articles by Hernandez, P. E.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Dec 1997, 4990-4992, Vol 63, No. 12
Copyright © 1997, American Society for Microbiology

One-step purification of nisin A by immunoaffinity chromatography

AM Suarez, JI Azcona, JM Rodriguez, B Sanz and PE Hernandez
Departamento de Nutricion y Bromatologia III, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain. anagea@eucmax.sim.ucm.es

The lantibiotic nisin A was purified to homogeneity by a single-step immunoaffinity chromatography method. An immunoadsorption matrix was developed by direct binding of anti-nisin A monoclonal antibodies to N- hydroxysuccinimide-activated Sepharose. The purification procedure was rapid and reproducible and rendered much higher final yields of nisin than any other described method.


This article has been cited by other articles:

  • Keren, T., Yarmus, M., Halevy, G., Shapira, R. (2004). Immunodetection of the Bacteriocin Lacticin RM: Analysis of the Influence of Temperature and Tween 80 on Its Expression and Activity. Appl. Environ. Microbiol. 70: 2098-2104 [Abstract] [Full Text]  
  • Richard, C., Drider, D., Fliss, I., Denery, S., Prevost, H. (2004). Generation and Utilization of Polyclonal Antibodies to a Synthetic C-Terminal Amino Acid Fragment of Divercin V41, a Class IIa Bacteriocin. Appl. Environ. Microbiol. 70: 248-254 [Abstract] [Full Text]  
  • Benech, R.-O., Kheadr, E. E., Laridi, R., Lacroix, C., Fliss, I. (2002). Inhibition of Listeria innocua in Cheddar Cheese by Addition of Nisin Z in Liposomes or by In Situ Production in Mixed Culture. Appl. Environ. Microbiol. 68: 3683-3690 [Abstract] [Full Text]  
  • Martínez, J. M., Kok, J., Sanders, J. W., Hernández, P. E. (2000). Heterologous Coproduction of Enterocin A and Pediocin PA-1 by Lactococcus lactis: Detection by Specific Peptide-Directed Antibodies. Appl. Environ. Microbiol. 66: 3543-3549 [Abstract] [Full Text]  
  • Martinez, J. M., Martinez, M. I., Herranz, C., Suarez, A., Fernandez, M. F., Cintas, L. M., Rodriguez, J. M., Hernandez, P. E. (1999). Antibodies to a synthetic 1-9-N-terminal amino acid fragment of mature pediocin PA-1: sensitivity and specificity for pediocin PA-1 and cross-reactivity against Class IIa bacteriocins. Microbiology 145: 2777-2787 [Abstract] [Full Text]  
  • Martínez, J. M., Martínez, M. I., Suárez, A. M., Herranz, C., Casaus, P., Cintas, L. M., Rodríguez, J. M., Hernández, P. E. (1998). Generation of Polyclonal Antibodies of Predetermined Specificity against Pediocin PA-1. Appl. Environ. Microbiol. 64: 4536-4545 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»