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Appl. Environ. Microbiol., Mar 1997, 1160-1164, Vol 63, No. 3
Copyright © 1997, American Society for Microbiology

Purification and Characterization of a Proteasome from the Hyperthermophilic Archaeon Pyrococcus furiosus

MW Bauer, SH Bauer and RM Kelly
Department of Chemical Engineering, North Carolina State University, Raleigh, North Carolina 27695-7905

A 640-kDa proteasome consisting of (alpha) (25-kDa) and (beta) (22-kDa) subunits, and with a temperature optimum of 95(deg)C, was purified from crude cell extracts of a hyperthermophilic archaeon, Pyrococcus furiosus. Although this is the fourth member of the kingdom Euryarchaeota (and the first hyperthermophile) found to contain a proteasome, none has been identified among the members of the kingdom Crenarchaeota.


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