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Appl. Environ. Microbiol., 03 1997, 1185-1188, Vol 63, No. 3
Copyright © 1997, American Society for Microbiology

Assessment of fecal bacteria with bile acid 7 alpha-dehydroxylating activity for the presence of bai-like genes

KC Doerner, F Takamine, CP LaVoie, DH Mallonee and PB Hylemon
Department of Microbiology and Immunology, Virginia Commonwealth University-Medical College of Virginia, Richmond 23298-0678, USA.

Eubacterium sp. strain VPI 12708 has several bile acid-inducible (bai) genes which encode enzymes in the bile acid 7 alpha-dehydroxylation (7 alpha DeOH) pathway. Twelve 7 alpha DeOH-positive intestinal bacterial strains were assayed for 7 alpha DeOH activity, and 13 strains were tested for hybridization with bai genes. Cholic acid 7 alpha DeOH activity varied greatly (> 100-fold) among these strains. Southern blot experiments showed that DNA prepared from 7 of 13 strains hybridized with at least one of the bai genes from Eubacterium sp. strain VPI 12708.


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