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Appl. Environ. Microbiol., Apr 1997, 1428-1433, Vol 63, No. 4
Copyright © 1997, American Society for Microbiology

Organic solvent tolerance and antibiotic resistance increased by overexpression of marA in Escherichia coli

H Asako, H Nakajima, K Kobayashi, M Kobayashi and R Aono
Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.

We previously reported that overexpression of the soxS or robA gene causes in several Escherichia coli strains the acquisition of higher organic solvent tolerance and also increased resistance to a number of antibiotics (H. Nakajima, K. Kobayashi, M. Kobayashi, H. Asako, and R. Aono, Appl. Environ. Microbiol. 61:2302-2307, 1995). Most E. coli strains cannot grow in the presence of cyclohexane. We isolated the marRAB genes from a Kohara lambda phage clone and cyclohexane-tolerant mutant strain OST3408. We found a substitution of serine for arginine at position 73 in the coding region of marR of OST3408 and designated the gene marR08. Our genetic analysis revealed that marR08 is responsible for the cyclohexane-tolerant phenotype. We observed that the marA gene on high-copy-number plasmids increased the organic solvent tolerance of E. coli strains. Furthermore, exposure of E. coli cells to salicylate, which activates the mar regulon genes, also raised organic solvent tolerance. Overexpression of the marA, soxS, or robA gene increased resistance to numerous antibiotics but not to hydrophilic aminoglycosides.




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Copyright © 1997 by the American Society for Microbiology. All rights reserved.