This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dubois, E.
Right arrow Articles by Pommepuy, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dubois, E.
Right arrow Articles by Pommepuy, M.
Agricola
Right arrow Articles by Dubois, E.
Right arrow Articles by Pommepuy, M.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., May 1997, 1794-1800, Vol 63, No. 5
Copyright © 1997, American Society for Microbiology

Molecular epidemiological survey of rotaviruses in sewage by reverse transcriptase seminested PCR and restriction fragment length polymorphism assay

E Dubois, F Le Guyader, L Haugarreau, H Kopecka, M Cormier and M Pommepuy
Laboratorie de Microbiologie, Institut Francais de Recherche pour l'Exploitation de la Mer, Nantes, France.

Rotavirus double-stranded RNA was detected directly in sewage treatment plant samples over a 1-year period by reverse transcription followed by PCR amplification of the VP7 gene and Southern blot hybridization. The presence of naturally occurring rotaviruses was demonstrated in 42% of raw sewage samples and in 67% of treated effluent samples. Amplified viral sequences were analyzed by restriction enzymes. Ten different restriction profiles were characterized, most of which were found in treated effluent samples. A mixture of restriction profiles was observed in 75% of contaminated effluent samples. The profiles were compared with those obtained from human rotavirus isolates involved in infections in children from the same area (six different profiles were detected). Five identical viral sequences were detected in both environmental and clinical samples. Restriction profiles were also compared to profiles from known genomic sequences of human and animal viruses. Both human and animal origins of rotavirus contamination of water seemed likely.


This article has been cited by other articles:

  • Blinkova, O., Rosario, K., Li, L., Kapoor, A., Slikas, B., Bernardin, F., Breitbart, M., Delwart, E. (2009). Frequent Detection of Highly Diverse Variants of Cardiovirus, Cosavirus, Bocavirus, and Circovirus in Sewage Samples Collected in the United States. J. Clin. Microbiol. 47: 3507-3513 [Abstract] [Full Text]  
  • Symonds, E. M., Griffin, D. W., Breitbart, M. (2009). Eukaryotic Viruses in Wastewater Samples from the United States. Appl. Environ. Microbiol. 75: 1402-1409 [Abstract] [Full Text]  
  • Banyai, K., Bogdan, A., Szucs, G., Arista, S., De Grazia, S., Kang, G., Banerjee, I., Iturriza-Gomara, M., Buonavoglia, C., Martella, V. (2009). Assignment of the group A rotavirus NSP4 gene into genotypes using a hemi-nested multiplex PCR assay: a rapid and reproducible assay for strain surveillance studies. J Med Microbiol 58: 303-311 [Abstract] [Full Text]  
  • Fong, T.-T., Lipp, E. K. (2005). Enteric Viruses of Humans and Animals in Aquatic Environments: Health Risks, Detection, and Potential Water Quality Assessment Tools. Microbiol. Mol. Biol. Rev. 69: 357-371 [Abstract] [Full Text]  
  • Queiroz, A. P. S., Santos, F. M., Sassaroli, A., Harsi, C. M., Monezi, T. A., Mehnert, D. U. (2001). Electropositive Filter Membrane as an Alternative for the Elimination of PCR Inhibitors from Sewage and Water Samples. Appl. Environ. Microbiol. 67: 4614-4618 [Abstract] [Full Text]  
  • Le Guyader, F., Haugarreau, L., Miossec, L., Dubois, E., Pommepuy, M. (2000). Three-Year Study To Assess Human Enteric Viruses in Shellfish. Appl. Environ. Microbiol. 66: 3241-3248 [Abstract] [Full Text]  
  • Gratacap-Cavallier, B., Genoulaz, O., Brengel-Pesce, K., Soule, H., Innocenti-Francillard, P., Bost, M., Gofti, L., Zmirou, D., Seigneurin, J. M. (2000). Detection of Human and Animal Rotavirus Sequences in Drinking Water. Appl. Environ. Microbiol. 66: 2690-2692 [Abstract] [Full Text]  
  • Vaitilingom, M., Gendre, F., Brignon, P. (1998). Direct Detection of Viable Bacteria, Molds, and Yeasts by Reverse Transcriptase PCR in Contaminated Milk Samples after Heat Treatment. Appl. Environ. Microbiol. 64: 1157-1160 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»