This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schloter, M. Articles by Hartmann, A. Search for Related Content PubMed PubMed Citation Articles by Schloter, M. Articles by Hartmann, A. Agricola Articles by Schloter, M. Articles by Hartmann, A.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., May 1997, 2038-2046, Vol 63, No. 5
Copyright © 1997, American Society for Microbiology

Root colonization of different plants by plant-growth-promoting Rhizobium leguminosarum bv. trifolii R39 studied with monospecific polyclonal antisera

M Schloter, W Wiehe, B Assmus, H Steindl, H Becke, G Hoflich and A Hartmann
Institute of Soil Ecology, GSF-National Research Center for Environment and Health, Neuherberg, Germany. schloter@gsf.de

Monospecific polyclonal antisera raised against Rhizobium leguminosarum bv. trifolii R39, a bacterium which was isolated originally from red clover nodules, were used to study the colonization of roots of leguminous and nonleguminous plants (Pisum sativum, Lupinus albus, Triticum aestivum, and Zea mays) after inoculation. Eight weeks after inoculation of soil-grown plants, between 0.1 and 1% of the total bacterial population in the rhizospheres of all inoculated plants were identified as R. leguminosarum bv. trifolii R39. To characterize the associative colonization of the nonleguminous plants by R.leguminosarum bv. trifolii R39 in more detail, a time course study was performed with inoculated roots of Z. mays. R. leguminosarum bv. trifolii R39 was found almost exclusively in the rhizosphere soil and on the rhizoplane 4 weeks after inoculation. Colonization of inner root tissues was detected only occasionally at this time. During the process of attachment of R. leguminosarum bv. trifolii R39 to the rhizoplane, bacterial lipopolysaccharides were overexpressed, and this may be important for plant-microbe interaction. Fourteen weeks after inoculation, microcolonies of R. leguminosarum bv. trifolii R39 were detected in lysed cells of the root cortex as well as in intracellular space of central root cylinder cells. At the beginning of flowering (18 weeks after inoculation), the number of R. leguminosarum bv. trifolii R39 organisms decreased in the rhizosphere soil, rhizoplane, and inner root tissue.

This article has been cited by other articles:

• Rinaudi, L. V., Gonzalez, J. E. (2009). The Low-Molecular-Weight Fraction of Exopolysaccharide II from Sinorhizobium meliloti Is a Crucial Determinant of Biofilm Formation. J. Bacteriol. 191: 7216-7224 [Abstract] [Full Text]  
• Peng, G., Yuan, Q., Li, H., Zhang, W., Tan, Z. (2008). Rhizobium oryzae sp. nov., isolated from the wild rice Oryza alta. Int. J. Syst. Evol. Microbiol. 58: 2158-2163 [Abstract] [Full Text]  
• Tan, Z., Hurek, T., Vinuesa, P., Muller, P., Ladha, J. K., Reinhold-Hurek, B. (2001). Specific Detection of Bradyrhizobium and Rhizobium Strains Colonizing Rice (Oryza sativa) Roots by 16S-23S Ribosomal DNA Intergenic Spacer-Targeted PCR. Appl. Environ. Microbiol. 67: 3655-3664 [Abstract] [Full Text]  
• Biswas, J. C., Ladha, J. K., Dazzo, F. B., Yanni, Y. G., Rolfe, B. G. (2000). Rhizobial Inoculation Influences Seedling Vigor and Yield of Rice. Agron. J. 92: 880-886 [Abstract] [Full Text]