This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vollmer, A. C.
Right arrow Articles by LaRossa, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vollmer, A. C.
Right arrow Articles by LaRossa, R. A.
Agricola
Right arrow Articles by Vollmer, A. C.
Right arrow Articles by LaRossa, R. A.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Jul 1997, 2566-2571, Vol 63, No. 7
Copyright © 1997, American Society for Microbiology

Detection of DNA damage by use of Escherichia coli carrying recA'::lux, uvrA'::lux, or alkA'::lux reporter plasmids

AC Vollmer, S Belkin, DR Smulski, TK Van Dyk and RA LaRossa
Swarthmore College, Pennsylvania 19081-1397, USA. avollme1@swarthmore.edu

Plasmids were constructed in which DNA damage-inducible promoters recA, uvrA, and alkA from Escherichia coli were fused to the Vibrio fischeri luxCDABE operon. Introduction of these plasmids into E. coli allowed the detection of a dose-dependent response to DNA-damaging agents, such as mitomycin and UV irradiation. Bioluminescence was measured in real time over extended periods. The fusion of the recA promoter to luxCDABE showed the most dramatic and sensitive responses. lexA dependence of the bioluminescent SOS response was demonstrated, confirming that this biosensor's reports were transmitted by the expected regulatory circuitry. Comparisons were made between luxCDABE and lacZ fusions to each promoter. It is suggested that the lux biosensors may have use in monitoring chemical, physical, and genotoxic agents as well as in further characterizing the mechanisms of DNA repair.


This article has been cited by other articles:

  • Soberon, N., Martin, R., Suarez, J. E. (2007). New Method for Evaluation of Genotoxicity, Based on the Use of Real-Time PCR and Lysogenic Gram-Positive and Gram-Negative Bacteria. Appl. Environ. Microbiol. 73: 2815-2819 [Abstract] [Full Text]  
  • Riedel, C. U., Monk, I. R., Casey, P. G., Morrissey, D., O'Sullivan, G. C., Tangney, M., Hill, C., Gahan, C. G. M. (2007). Improved Luciferase Tagging System for Listeria monocytogenes Allows Real-Time Monitoring In Vivo and In Vitro. Appl. Environ. Microbiol. 73: 3091-3094 [Abstract] [Full Text]  
  • Bron, P. A., Monk, I. R., Corr, S. C., Hill, C., Gahan, C. G. M. (2006). Novel Luciferase Reporter System for In Vitro and Organ-Specific Monitoring of Differential Gene Expression in Listeria monocytogenes. Appl. Environ. Microbiol. 72: 2876-2884 [Abstract] [Full Text]  
  • Wiles, S., Ferguson, K., Stefanidou, M., Young, D. B., Robertson, B. D. (2005). Alternative Luciferase for Monitoring Bacterial Cells under Adverse Conditions. Appl. Environ. Microbiol. 71: 3427-3432 [Abstract] [Full Text]  
  • Norman, A., Hestbjerg Hansen, L., Sorensen, S. J. (2005). Construction of a ColD cda Promoter-Based SOS-Green Fluorescent Protein Whole-Cell Biosensor with Higher Sensitivity toward Genotoxic Compounds than Constructs Based on recA, umuDC, or sulA Promoters. Appl. Environ. Microbiol. 71: 2338-2346 [Abstract] [Full Text]  
  • Kobayashi, H., Kaern, M., Araki, M., Chung, K., Gardner, T. S., Cantor, C. R., Collins, J. J. (2004). Programmable cells: Interfacing natural and engineered gene networks. Proc. Natl. Acad. Sci. USA 101: 8414-8419 [Abstract] [Full Text]  
  • Palma, M., DeLuca, D., Worgall, S., Quadri, L. E. N. (2004). Transcriptome Analysis of the Response of Pseudomonas aeruginosa to Hydrogen Peroxide. J. Bacteriol. 186: 248-252 [Abstract] [Full Text]  
  • Kim, Y.-H., Lee, J., Ahn, J.-Y., Gu, M. B., Moon, S.-H. (2002). Enhanced Degradation of an Endocrine-Disrupting Chemical, Butyl Benzyl Phthalate, by Fusarium oxysporum f. sp. pisi Cutinase. Appl. Environ. Microbiol. 68: 4684-4688 [Abstract] [Full Text]  
  • Shapiro, E., Baneyx, F. (2002). Stress-Based Identification and Classification of Antibacterial Agents: Second-Generation Escherichia coli Reporter Strains and Optimization of Detection. Antimicrob. Agents Chemother. 46: 2490-2497 [Abstract] [Full Text]  
  • Smulski, D. R., Huang, L. L., McCluskey, M. P., Reeve, M. J. G., Vollmer, A. C., Van Dyk, T. K., LaRossa, R. A. (2001). Combined, Functional Genomic-Biochemical Approach to Intermediary Metabolism: Interaction of Acivicin, a Glutamine Amidotransferase Inhibitor, with Escherichia coli K-12. J. Bacteriol. 183: 3353-3364 [Abstract] [Full Text]  
  • Wei, Y., Vollmer, A. C., LaRossa, R. A. (2001). In Vivo Titration of Mitomycin C Action by Four Escherichia coli Genomic Regions on Multicopy Plasmids. J. Bacteriol. 183: 2259-2264 [Abstract] [Full Text]  
  • Hansen, L. H., Ferrari, B., Sørensen, A. H., Veal, D., Sørensen, S. J. (2001). Detection of Oxytetracycline Production by Streptomyces rimosus in Soil Microcosms by Combining Whole-Cell Biosensors and Flow Cytometry. Appl. Environ. Microbiol. 67: 239-244 [Abstract] [Full Text]  
  • Mason, C. A., Dünner, J., Indra, P., Colangelo, T. (1999). Heat-Induced Expression and Chemically Induced Expression of the Escherichia coli Stress Protein HtpG Are Affected by the Growth Environment. Appl. Environ. Microbiol. 65: 3433-3440 [Abstract] [Full Text]  
  • Elasri, M. O., Miller, R. V. (1999). Study of the Response of a Biofilm Bacterial Community to UV Radiation. Appl. Environ. Microbiol. 65: 2025-2031 [Abstract] [Full Text]  
  • Vollmer, A. C., Kwakye, S., Halpern, M., Everbach, E. C. (1998). Bacterial Stress Responses to 1-Megahertz Pulsed Ultrasound in the Presence of Microbubbles. Appl. Environ. Microbiol. 64: 3927-3931 [Abstract] [Full Text]  
  • Van Dyk, T. K., Ayers, B. L., Morgan, R. W., Larossa, R. A. (1998). Constricted Flux through the Branched-Chain Amino Acid Biosynthetic Enzyme Acetolactate Synthase Triggers Elevated Expression of Genes Regulated by rpoS and Internal Acidification. J. Bacteriol. 180: 785-792 [Abstract] [Full Text]  
  • Van Dyk, T. K., Wei, Y., Hanafey, M. K., Dolan, M., Reeve, M. J. G., Rafalski, J. A., Rothman-Denes, L. B., LaRossa, R. A. (2001). A genomic approach to gene fusion technology. Proc. Natl. Acad. Sci. USA 98: 2555-2560 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»