This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bowman, J. P.
Right arrow Articles by McMeekin, T. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bowman, J. P.
Right arrow Articles by McMeekin, T. A.
Agricola
Right arrow Articles by Bowman, J. P.
Right arrow Articles by McMeekin, T. A.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 08 1997, 3068-3078, Vol 63, No. 8
Copyright © 1997, American Society for Microbiology

Diversity and association of psychrophilic bacteria in Antarctic sea ice

JP Bowman, SA McCammon, MV Brown, DS Nichols and TA McMeekin
Antarctic CRC, University of Tasmania, Hobart, Tasmania, Australia. john.bowman@.utas.edu.au

The bacterial populations associated with sea ice sampled from Antarctic coastal areas were investigated by use of a phenotypic approach and a phylogenetic approach based on genes encoding 16S rRNA (16S rDNA). The diversity of bacteria associated with sea ice was also compared with the bacterial diversity of seawater underlying sea ice. Psychrophilic (optimal growth temperature, < or = 15 degrees C; no growth occurring at 20 degrees C) bacterial diversity was found to be significantly enriched in sea ice samples possessing platelet and bottom ice diatom assemblages, with 2 to 9 distinct (average, 5.6 +/- 1.8) psychrophilic taxa isolated per sample. Substantially fewer psychrophilic isolates were recovered from ice cores with a low or negligible population of ice diatoms or from under-ice seawater samples (less than one distinct taxon isolated per sample). In addition, psychrophilic taxa that were isolated from under-ice seawater samples were in general phylogenetically distinct from psychrophilic taxa isolated from sea ice cores. The taxonomic distributions of psychrotrophic bacterial isolates (optimal growth temperature, > 20 degrees C; growth can occur at approximately 4 degrees C) isolated from sea ice cores and under-ice seawater were quite similar. Overall, bacterial isolates from Antarctic sea ice were found to belong to four phylogenetic groups, the alpha and gamma subdivisions of the Proteobacteria, the gram-positive branch, and the Flexibacter- Bacteroides-Cytophaga phylum. Most of the sea ice strains examined appeared to be novel taxa based on phylogenetic comparisons, with 45% of the strains being psychrophilic. 16S rDNA sequence analysis revealed that psychrophilic strains belonged to the genera Colwellia, Shewanella, Marinobacter, Planococcus, and novel phylogenetic lineages adjacent to Colwellia and Alteromonas and within the Flexibacter- Bacteroides-Cytophaga phylum. Psychrotrophic strains were found to be members of the genera Pseudoalteromonas, Psychrobacter, Halomonas, Pseudomonas, Hyphomonas, Sphingomonas, Arthrobacter, Planococcus, and Halobacillus. From this survey, it is proposed that ice diatom assemblages provide niches conducive to the proliferation of a diverse array of psychrophilic bacterial species.


This article has been cited by other articles:

  • Shiratori, H., Tagami, Y., Morishita, T., Kamihara, Y., Beppu, T., Ueda, K. (2009). Filimonas lacunae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from fresh water. Int. J. Syst. Evol. Microbiol. 59: 1137-1142 [Abstract] [Full Text]  
  • Byrne-Bailey, K. G., Gaze, W. H., Kay, P., Boxall, A. B. A., Hawkey, P. M., Wellington, E. M. H. (2009). Prevalence of Sulfonamide Resistance Genes in Bacterial Isolates from Manured Agricultural Soils and Pig Slurry in the United Kingdom. Antimicrob. Agents Chemother. 53: 696-702 [Abstract] [Full Text]  
  • Tang, K. W, Smith, W. O Jr, Shields, A. R, Elliott, D. T (2009). Survival and recovery of Phaeocystis antarctica (Prymnesiophyceae) from prolonged darkness and freezing. Proc R Soc B 276: 81-90 [Abstract] [Full Text]  
  • Bruckner, C. G., Bahulikar, R., Rahalkar, M., Schink, B., Kroth, P. G. (2008). Bacteria Associated with Benthic Diatoms from Lake Constance: Phylogeny and Influences on Diatom Growth and Secretion of Extracellular Polymeric Substances. Appl. Environ. Microbiol. 74: 7740-7749 [Abstract] [Full Text]  
  • Bercovich, A., Vazquez, S. C., Yankilevich, P., Coria, S. H., Foti, M., Hernandez, E., Vidal, A., Ruberto, L., Melo, C., Marenssi, S., Criscuolo, M., Memoli, M., Arguelles, M., Mac Cormack, W. P. (2008). Bizionia argentinensis sp. nov., isolated from surface marine water in Antarctica. Int. J. Syst. Evol. Microbiol. 58: 2363-2367 [Abstract] [Full Text]  
  • Yoon, J., Matsuo, Y., Kasai, H., Yokota, A. (2008). Limibacter armeniacum gen. nov., sp. nov., a novel representative of the family 'Flammeovirgaceae' isolated from marine sediment. Int. J. Syst. Evol. Microbiol. 58: 982-986 [Abstract] [Full Text]  
  • An, D.-S., Lee, H.-G., Im, W.-T., Liu, Q.-M., Lee, S.-T. (2007). Segetibacter koreensis gen. nov., sp. nov., a novel member of the phylum Bacteroidetes, isolated from the soil of a ginseng field in South Korea. Int. J. Syst. Evol. Microbiol. 57: 1828-1833 [Abstract] [Full Text]  
  • Yoon, J., Ishikawa, S., Kasai, H., Yokota, A. (2007). Perexilibacter aurantiacus gen. nov., sp. nov., a novel member of the family 'Flammeovirgaceae' isolated from sediment. Int. J. Syst. Evol. Microbiol. 57: 964-968 [Abstract] [Full Text]  
  • Katayama, T., Tanaka, M., Moriizumi, J., Nakamura, T., Brouchkov, A., Douglas, T. A., Fukuda, M., Tomita, F., Asano, K. (2007). Phylogenetic Analysis of Bacteria Preserved in a Permafrost Ice Wedge for 25,000 Years. Appl. Environ. Microbiol. 73: 2360-2363 [Abstract] [Full Text]  
  • Moss, J. A., Nocker, A., Lepo, J. E., Snyder, R. A. (2006). Stability and Change in Estuarine Biofilm Bacterial Community Diversity. Appl. Environ. Microbiol. 72: 5679-5688 [Abstract] [Full Text]  
  • Suresh, K., Mayilraj, S., Chakrabarti, T. (2006). Effluviibacter roseus gen. nov., sp. nov., isolated from muddy water, belonging to the family 'Flexibacteraceae'. Int. J. Syst. Evol. Microbiol. 56: 1703-1707 [Abstract] [Full Text]  
  • Walker, V. K., Palmer, G. R., Voordouw, G. (2006). Freeze-thaw tolerance and clues to the winter survival of a soil community.. Appl. Environ. Microbiol. 72: 1784-1792 [Abstract] [Full Text]  
  • O'Sullivan, L. A., Rinna, J., Humphreys, G., Weightman, A. J., Fry, J. C. (2006). Culturable phylogenetic diversity of the phylum 'Bacteroidetes' from river epilithon and coastal water and description of novel members of the family Flavobacteriaceae: Epilithonimonas tenax gen. nov., sp. nov. and Persicivirga xylanidelens gen. nov., sp. nov.. Int. J. Syst. Evol. Microbiol. 56: 169-180 [Abstract] [Full Text]  
  • Yoon, J.-H., Kang, S.-J., Lee, C.-H., Oh, T.-K. (2006). Donghaeana dokdonensis gen. nov., sp. nov., isolated from sea water. Int. J. Syst. Evol. Microbiol. 56: 187-191 [Abstract] [Full Text]  
  • Lozupone, C., Knight, R. (2005). UniFrac: a New Phylogenetic Method for Comparing Microbial Communities. Appl. Environ. Microbiol. 71: 8228-8235 [Abstract] [Full Text]  
  • Yoon, J.-H., Kang, S.-J., Lee, C.-H., Oh, T.-K. (2005). Dokdonia donghaensis gen. nov., sp. nov., isolated from sea water. Int. J. Syst. Evol. Microbiol. 55: 2323-2328 [Abstract] [Full Text]  
  • Kaartokallio, H., Laamanen, M., Sivonen, K. (2005). Responses of Baltic Sea Ice and Open-Water Natural Bacterial Communities to Salinity Change. Appl. Environ. Microbiol. 71: 4364-4371 [Abstract] [Full Text]  
  • Nichols, C. M., Bowman, J. P., Guezennec, J. (2005). Effects of Incubation Temperature on Growth and Production of Exopolysaccharides by an Antarctic Sea Ice Bacterium Grown in Batch Culture. Appl. Environ. Microbiol. 71: 3519-3523 [Abstract] [Full Text]  
  • Bowman, J. P., Nichols, D. S. (2005). Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia. Int. J. Syst. Evol. Microbiol. 55: 1471-1486 [Abstract] [Full Text]  
  • Pujalte, M. J., Macian, M. C., Arahal, D. R., Ludwig, W., Schleifer, K. H., Garay, E. (2005). Nereida ignava gen. nov., sp. nov., a novel aerobic marine {alpha}-proteobacterium that is closely related to uncultured Prionitis (alga) gall symbionts. Int. J. Syst. Evol. Microbiol. 55: 631-636 [Abstract] [Full Text]  
  • Nedashkovskaya, O. I., Kim, S. B., Lee, K. H., Bae, K. S., Frolova, G. M., Mikhailov, V. V., Kim, I. S. (2005). Pibocella ponti gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi. Int. J. Syst. Evol. Microbiol. 55: 177-181 [Abstract] [Full Text]  
  • Nelson, K. E., Methe, B. (2005). Metabolism and Genomics: Adventures Derived From Complete Genome Sequencing. Reviews in Mineralogy and Geochemistry 59: 279-294 [Full Text]  
  • Van Trappen, S., Vandecandelaere, I., Mergaert, J., Swings, J. (2004). Algoriphagus antarcticus sp. nov., a novel psychrophile from microbial mats in Antarctic lakes. Int. J. Syst. Evol. Microbiol. 54: 1969-1973 [Abstract] [Full Text]  
  • Nocker, A., Lepo, J. E., Snyder, R. A. (2004). Influence of an Oyster Reef on Development of the Microbial Heterotrophic Community of an Estuarine Biofilm. Appl. Environ. Microbiol. 70: 6834-6845 [Abstract] [Full Text]  
  • Romanenko, L. A., Lysenko, A. M., Rohde, M., Mikhailov, V. V., Stackebrandt, E. (2004). Psychrobacter maritimus sp. nov. and Psychrobacter arenosus sp. nov., isolated from coastal sea ice and sediments of the Sea of Japan. Int. J. Syst. Evol. Microbiol. 54: 1741-1745 [Abstract] [Full Text]  
  • Van Trappen, S., Vandecandelaere, I., Mergaert, J., Swings, J. (2004). Gillisia limnaea gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from a microbial mat in Lake Fryxell, Antarctica. Int. J. Syst. Evol. Microbiol. 54: 445-448 [Abstract] [Full Text]  
  • Kaye, J. Z., Marquez, M. C., Ventosa, A., Baross, J. A. (2004). Halomonas neptunia sp. nov., Halomonas sulfidaeris sp. nov., Halomonas axialensis sp. nov. and Halomonas hydrothermalis sp. nov.: halophilic bacteria isolated from deep-sea hydrothermal-vent environments. Int. J. Syst. Evol. Microbiol. 54: 499-511 [Abstract] [Full Text]  
  • Miteva, V. I., Sheridan, P. P., Brenchley, J. E. (2004). Phylogenetic and Physiological Diversity of Microorganisms Isolated from a Deep Greenland Glacier Ice Core. Appl. Environ. Microbiol. 70: 202-213 [Abstract] [Full Text]  
  • Yumoto, I., Hirota, K., Sogabe, Y., Nodasaka, Y., Yokota, Y., Hoshino, T. (2003). Psychrobacter okhotskensis sp. nov., a lipase-producing facultative psychrophile isolated from the coast of the Okhotsk Sea. Int. J. Syst. Evol. Microbiol. 53: 1985-1989 [Abstract] [Full Text]  
  • Brinkmeyer, R., Knittel, K., Jurgens, J., Weyland, H., Amann, R., Helmke, E. (2003). Diversity and Structure of Bacterial Communities in Arctic versus Antarctic Pack Ice. Appl. Environ. Microbiol. 69: 6610-6619 [Abstract] [Full Text]  
  • Bowman, J. P., Nichols, C. M., Gibson, J. A. E. (2003). Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int. J. Syst. Evol. Microbiol. 53: 1343-1355 [Abstract] [Full Text]  
  • Ah Tow, L., Cowan, D. A. (2003). Non-specificity of Staphylococcus generic primers. Microbiology 149: 1605-1607 [Full Text]  
  • Bowman, J. P., McCammon, S. A., Gibson, J. A. E., Robertson, L., Nichols, P. D. (2003). Prokaryotic Metabolic Activity and Community Structure in Antarctic Continental Shelf Sediments. Appl. Environ. Microbiol. 69: 2448-2462 [Abstract] [Full Text]  
  • Vela, A. I., Collins, M. D., Latre, M. V., Mateos, A., Moreno, M. A., Hutson, R., Dominguez, L., Fernandez-Garayzabal, J. F. (2003). Psychrobacter pulmonis sp. nov., isolated from the lungs of lambs. Int. J. Syst. Evol. Microbiol. 53: 415-419 [Abstract] [Full Text]  
  • Tamaki, H., Hanada, S., Kamagata, Y., Nakamura, K., Nomura, N., Nakano, K., Matsumura, M. (2003). Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments. Int. J. Syst. Evol. Microbiol. 53: 519-526 [Abstract] [Full Text]  
  • Groudieva, T., Grote, R., Antranikian, G. (2003). Psychromonas arctica sp. nov., a novel psychrotolerant, biofilm-forming bacterium isolated from Spitzbergen. Int. J. Syst. Evol. Microbiol. 53: 539-545 [Abstract] [Full Text]  
  • Wery, N., Gerike, U., Sharman, A., Chaudhuri, J. B., Hough, D. W., Danson, M. J. (2003). Use of a Packed-Column Bioreactor for Isolation of Diverse Protease-Producing Bacteria from Antarctic Soil. Appl. Environ. Microbiol. 69: 1457-1464 [Abstract] [Full Text]  
  • Flagan, S., Ching, W.-K., Leadbetter, J. R. (2003). Arthrobacter Strain VAI-A Utilizes Acyl-Homoserine Lactone Inactivation Products and Stimulates Quorum Signal Biodegradation by Variovorax paradoxus. Appl. Environ. Microbiol. 69: 909-916 [Abstract] [Full Text]  
  • Morris, C. E., Bardin, M., Berge, O., Frey-Klett, P., Fromin, N., Girardin, H., Guinebretiere, M.-H., Lebaron, P., Thiery, J. M., Troussellier, M. (2002). Microbial Biodiversity: Approaches to Experimental Design and Hypothesis Testing in Primary Scientific Literature from 1975 to 1999. Microbiol. Mol. Biol. Rev. 66: 592-616 [Abstract] [Full Text]  
  • Christner, B. C. (2002). Incorporation of DNA and Protein Precursors into Macromolecules by Bacteria at -15oC. Appl. Environ. Microbiol. 68: 6435-6438 [Abstract] [Full Text]  
  • Pinar, G., Ramos, C., Rolleke, S., Schabereiter-Gurtner, C., Vybiral, D., Lubitz, W., Denner, E. B. M. (2001). Detection of Indigenous Halobacillus Populations in Damaged Ancient Wall Paintings and Building Materials: Molecular Monitoring and Cultivation. Appl. Environ. Microbiol. 67: 4891-4895 [Abstract] [Full Text]  
  • Madrid, V. M., Taylor, G. T., Scranton, M. I., Chistoserdov, A. Y. (2001). Phylogenetic Diversity of Bacterial and Archaeal Communities in the Anoxic Zone of the Cariaco Basin. Appl. Environ. Microbiol. 67: 1663-1674 [Abstract] [Full Text]  
  • Winter, C., Moeseneder, M. M., Herndl, G. J. (2001). Impact of UV Radiation on Bacterioplankton Community Composition. Appl. Environ. Microbiol. 67: 665-672 [Abstract] [Full Text]  
  • Battin, T. J., Wille, A., Sattler, B., Psenner, R. (2001). Phylogenetic and Functional Heterogeneity of Sediment Biofilms along Environmental Gradients in a Glacial Stream. Appl. Environ. Microbiol. 67: 799-807 [Abstract] [Full Text]  
  • Barbieri, E., Potenza, L., Rossi, I., Sisti, D., Giomaro, G., Rossetti, S., Beimfohr, C., Stocchi, V. (2000). Phylogenetic Characterization and In Situ Detection of a Cytophaga-Flexibacter-Bacteroides Phylogroup Bacterium in Tuber borchii Vittad. Ectomycorrhizal Mycelium. Appl. Environ. Microbiol. 66: 5035-5042 [Abstract] [Full Text]  
  • Teske, A., Brinkhoff, T., Muyzer, G., Moser, D. P., Rethmeier, J., Jannasch, H. W. (2000). Diversity of Thiosulfate-Oxidizing Bacteria from Marine Sediments and Hydrothermal Vents. Appl. Environ. Microbiol. 66: 3125-3133 [Abstract] [Full Text]  
  • Eilers, H., Pernthaler, J., Glöckner, F. O., Amann, R. (2000). Culturability and In Situ Abundance of Pelagic Bacteria from the North Sea. Appl. Environ. Microbiol. 66: 3044-3051 [Abstract] [Full Text]  
  • Nichols, D. S., Olley, J., Garda, H., Brenner, R. R., McMeekin, T. A. (2000). Effect of Temperature and Salinity Stress on Growth and Lipid Composition of Shewanella gelidimarina. Appl. Environ. Microbiol. 66: 2422-2429 [Abstract] [Full Text]  
  • Sheridan, P. P., Brenchley, J. E. (2000). Characterization of a Salt-Tolerant Family 42 beta -Galactosidase from a Psychrophilic Antarctic Planococcus Isolate. Appl. Environ. Microbiol. 66: 2438-2444 [Abstract] [Full Text]  
  • Cho, J.-C., Kim, S.-J. (2000). Increase in Bacterial Community Diversity in Subsurface Aquifers Receiving Livestock Wastewater Input. Appl. Environ. Microbiol. 66: 956-965 [Abstract] [Full Text]  
  • Riemann, L., Steward, G. F., Azam, F. (2000). Dynamics of Bacterial Community Composition and Activity during a Mesocosm Diatom Bloom. Appl. Environ. Microbiol. 66: 578-587 [Abstract] [Full Text]  
  • Nichols, D. S., Greenhill, A. R., Shadbolt, C. T., Ross, T., McMeekin, T. A. (1999). Physicochemical Parameters for Growth of the Sea Ice Bacteria Glaciecola punicea ACAM 611T and Gelidibacter sp. Strain IC158. Appl. Environ. Microbiol. 65: 3757-3760 [Abstract] [Full Text]  
  • Murray, A. E., Preston, C. M., Massana, R., Taylor, L. T., Blakis, A., Wu, K., DeLong, E. F. (1998). Seasonal and Spatial Variability of Bacterial and Archaeal Assemblages in the Coastal Waters near Anvers Island, Antarctica. Appl. Environ. Microbiol. 64: 2585-2595 [Abstract] [Full Text]  
  • Llobet-Brossa, E., Rosselló-Mora, R., Amann, R. (1998). Microbial Community Composition of Wadden Sea Sediments as Revealed by Fluorescence In Situ Hybridization. Appl. Environ. Microbiol. 64: 2691-2696 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»