This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Herman, L. M. Articles by Waes, G. M. Search for Related Content PubMed PubMed Citation Articles by Herman, L. M. Articles by Waes, G. M. Agricola Articles by Herman, L. M. Articles by Waes, G. M.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 08 1997, 3139-3143, Vol 63, No. 8
Copyright © 1997, American Society for Microbiology

Identification and detection of Bacillus sporothermodurans spores in 1, 10, and 100 milliliters of raw milk by PCR

LM Herman, MJ Vaerewijck, RJ Moermans and GM Waes
Government Dairy Research Station, Agricultural Centre Ghent, Melle, Belgium.

A PCR method was developed to detect spores of Bacillus sporothermodurans in 1, 10, and 100 ml of raw milk. Two primers were derived from a unique sequence after subtractive hybridization of B. sporothermodurans DNA with DNA of MB 397, a not yet identified spore- forming bacterium isolated from raw milk, closely related to B. sporothermodurans. Specific identification was proven on a large collection of Bacillus strains and on strains from relevant taxa. The detection of B. sporothermodurans in raw milk is based on activation, germination, and outgrowth of the spores, followed by PCR identification. Spores from 10 and 100 ml were concentrated by centrifugation after chemical extraction of the milk components. The total test takes 28 h. The detection limits are 9, 0.4, and 0.22 CFU/ml for 1, 10, and 100 ml, respectively.

This article has been cited by other articles:

• Scheldeman, P., Pil, A., Herman, L., De Vos, P., Heyndrickx, M. (2005). Incidence and Diversity of Potentially Highly Heat-Resistant Spores Isolated at Dairy Farms. Appl. Environ. Microbiol. 71: 1480-1494 [Abstract] [Full Text]  
• Scheldeman, P., Goossens, K., Rodriguez-Diaz, M., Pil, A., Goris, J., Herman, L., De Vos, P., Logan, N. A., Heyndrickx, M. (2004). Paenibacillus lactis sp. nov., isolated from raw and heat-treated milk. Int. J. Syst. Evol. Microbiol. 54: 885-891 [Abstract] [Full Text]