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Appl. Environ. Microbiol., Aug 1997, 3301-3303, Vol 63, No. 8
Copyright © 1997, American Society for Microbiology

Interference of peptone and tyrosine with the lignin peroxidase assay

R ten Have, S Hartmans and JA Field
Department of Food Science, Wageningen Agricultural University, The Netherlands. Rimko.tenHave@algemeen.im.wau.nl

The N-unregulated white rot fungus Bjerkandera sp. strain BOS55 was cultured in 1 liter of peptone-yeast extract medium to produce lignin peroxidase (LiP). During the LiP assay, the oxidation of veratryl alcohol to veratraldehyde was inhibited due to tyrosine present in the peptone and the yeast extract.


This article has been cited by other articles:

  • Caramelo, L., Martínez, M. J., Martínez, A. T. (1999). A Search for Ligninolytic Peroxidases in the Fungus Pleurotus eryngii Involving alpha -Keto-gamma -Thiomethylbutyric Acid and Lignin Model Dimers. Appl. Environ. Microbiol. 65: 916-922 [Abstract] [Full Text]  


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