This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Scott, K. P.
Right arrow Articles by Flint, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Scott, K. P.
Right arrow Articles by Flint, H. J.
Agricola
Right arrow Articles by Scott, K. P.
Right arrow Articles by Flint, H. J.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 09 1997, 3405-3411, Vol 63, No. 9
Copyright © 1997, American Society for Microbiology

High-frequency transfer of a naturally occurring chromosomal tetracycline resistance element in the ruminal anaerobe Butyrivibrio fibrisolvens

KP Scott, TM Barbosa, KJ Forbes and HJ Flint
Rowett Research Institute, University of Aberdeen, United Kingdom.

Butyrivibrio fibrisolvens strains resistant to tetracycline were isolated from the bovine rumen. Two of three Tcr B. fibrisolvens tested were able to donate tetracycline resistance at frequencies ranging from 10(-7) to 10(-1) per donor cell in anaerobic filter matings to a rifampin-resistant mutant of the type strain of B.fibrisolvens, 2221R. The recipient strain 2221R exhibited rapid autoaggregation, which might be a factor in the high transfer rates observed. Tcr transconjugants of B. fibrisolvens 2221R were also capable of further transferring tetracycline resistance to a fusidic acid-resistant mutant, 2221F. Comparison of genomic DNAs by pulsed-field gel electrophoresis demonstrated altered band profiles in transconjugants, consistent with the acquisition of a large mobile chromosomal element. The transferable elements from the two B. fibrisolvens donors 1.23 and 1.230 (TnB123 and TnB1230, respectively) showed the same preferred insertion site in the B. fibrisolvens 2221R chromosome and are likely to be similar, or identical, elements. Hybridization experiments showed no close relationship between TnB1230 and int-xis regions from Tn916 or Tn5253. Although DNA from the B. fibrisolvens donor strains hybridized with probes carrying tet(M) or tet(O) sequences, transconjugants were found to have acquired a distinct band that hybridized only weakly with these probes, suggesting that a second, distantly related Tcr determinant had been transferred.


This article has been cited by other articles:

  • Kazimierczak, K. A., Scott, K. P., Kelly, D., Aminov, R. I. (2009). Tetracycline Resistome of the Organic Pig Gut. Appl. Environ. Microbiol. 75: 1717-1722 [Abstract] [Full Text]  
  • Hunter, P. R., Wilkinson, D. C., Catling, L. A., Barker, G. C. (2008). Meta-Analysis of Experimental Data Concerning Antimicrobial Resistance Gene Transfer Rates during Conjugation. Appl. Environ. Microbiol. 74: 6085-6090 [Abstract] [Full Text]  
  • Scott, K. P., Martin, J. C., Mrazek, J., Flint, H. J. (2008). Transfer of Conjugative Elements from Rumen and Human Firmicutes Bacteria to Roseburia inulinivorans. Appl. Environ. Microbiol. 74: 3915-3917 [Abstract] [Full Text]  
  • Spigaglia, P., Barbanti, F., Mastrantonio, P. (2008). Tetracycline Resistance Gene tet(W) in the Pathogenic Bacterium Clostridium difficile. Antimicrob. Agents Chemother. 52: 770-773 [Abstract] [Full Text]  
  • Storteboom, H. N., Kim, S.-C., Doesken, K. C., Carlson, K. H., Davis, J. G., Pruden, A. (2007). Response of Antibiotics and Resistance Genes to High-Intensity and Low-Intensity Manure Management. J. Environ. Qual. 36: 1695-1703 [Abstract] [Full Text]  
  • Florez, A. B., Ammor, M. S., Alvarez-Martin, P., Margolles, A., Mayo, B. (2006). Molecular Analysis of tet(W) Gene-Mediated Tetracycline Resistance in Dominant Intestinal Bifidobacterium Species from Healthy Humans. Appl. Environ. Microbiol. 72: 7377-7379 [Abstract] [Full Text]  
  • Billington, S. J., Jost, B. H. (2006). Multiple Genetic Elements Carry the Tetracycline Resistance Gene tet(W) in the Animal Pathogen Arcanobacterium pyogenes. Antimicrob. Agents Chemother. 50: 3580-3587 [Abstract] [Full Text]  
  • Kazimierczak, K. A., Flint, H. J., Scott, K. P. (2006). Comparative Analysis of Sequences Flanking tet(W) Resistance Genes in Multiple Species of Gut Bacteria.. Antimicrob. Agents Chemother. 50: 2632-2639 [Abstract] [Full Text]  
  • Masco, L., Van Hoorde, K., De Brandt, E., Swings, J., Huys, G. (2006). Antimicrobial susceptibility of Bifidobacterium strains from humans, animals and probiotic products. J Antimicrob Chemother 58: 85-94 [Abstract] [Full Text]  
  • Spigaglia, P., Barbanti, F., Mastrantonio, P. (2005). Horizontal Transfer of Erythromycin Resistance from Clostridium difficile to Butyrivibrio fibrisolvens. Antimicrob. Agents Chemother. 49: 5142-5145 [Abstract] [Full Text]  
  • Barbosa, T. M., Serra, C. R., La Ragione, R. M., Woodward, M. J., Henriques, A. O. (2005). Screening for Bacillus Isolates in the Broiler Gastrointestinal Tract. Appl. Environ. Microbiol. 71: 968-978 [Abstract] [Full Text]  
  • Melville, C. M., Brunel, R., Flint, H. J., Scott, K. P. (2004). The Butyrivibrio fibrisolvens tet(W) Gene Is Carried on the Novel Conjugative Transposon TnB1230, Which Contains Duplicated Nitroreductase Coding Sequences. J. Bacteriol. 186: 3656-3659 [Abstract] [Full Text]  
  • Billington, S. J., Songer, J. G., Jost, B. H. (2002). Widespread Distribution of a Tet W Determinant among Tetracycline-Resistant Isolates of the Animal Pathogen Arcanobacterium pyogenes. Antimicrob. Agents Chemother. 46: 1281-1287 [Abstract] [Full Text]  
  • Melville, C. M., Scott, K. P., Mercer, D. K., Flint, H. J. (2001). Novel Tetracycline Resistance Gene, tet(32), in the Clostridium-Related Human Colonic Anaerobe K10 and Its Transmission In Vitro to the Rumen Anaerobe Butyrivibrio fibrisolvens. Antimicrob. Agents Chemother. 45: 3246-3249 [Abstract] [Full Text]  
  • Whittle, G., Hund, B. D., Shoemaker, N. B., Salyers, A. A. (2001). Characterization of the 13-Kilobase ermF Region of the Bacteroides Conjugative Transposon CTnDOT. Appl. Environ. Microbiol. 67: 3488-3495 [Abstract] [Full Text]  
  • Frischer, M. E., Danforth, J. M., Healy, M. A. N., Saunders, F. M. (2000). Whole-Cell versus Total RNA Extraction for Analysis of Microbial Community Structure with 16S rRNA-Targeted Oligonucleotide Probes in Salt Marsh Sediments. Appl. Environ. Microbiol. 66: 3037-3043 [Abstract] [Full Text]  
  • Scott, K. P., Melville, C. M., Barbosa, T. M., Flint, H. J. (2000). Occurrence of the New Tetracycline Resistance Gene tet(W) in Bacteria from the Human Gut. Antimicrob. Agents Chemother. 44: 775-777 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»