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Appl Environ Microbiol, January 1998, p. 133-137, Vol. 64, No. 1
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Morphological and Molecular Identification of Trichoderma Isolates on North American Mushroom Farms

Alan Castle,^1,* Donna Speranzini,^1, Nezar Rghei,¹ Glen Alm,² Dan Rinker,² and John Bissett³

Brock University, St. Catharines, Ontario, Canada L2S 3A1,¹ Horticultural Research Institute of Ontario, Vineland Station, Ontario, Canada L0R 2E0,² and Eastern Cereal and Oilseed Research Centre, Central Experimental Farm, Ottawa, Ontario, Canada K1A 0C6³

Received 27 December 1996/Accepted 16 October 1997

Green mold disease (causal agent, Trichoderma) has resulted in severe crop losses on mushroom farms worldwide in recent years. We analyzed 160 isolates of Trichoderma from mushroom farms for morphological, cultural, and molecular characteristics and classified these isolates into phenotypic groups. The most common group comprised approximately 40% of the isolates and was identified as a strain of Trichoderma harzianum. This group was consistently recovered from farms with severe green mold disease but not from farms with little or no problem. In addition, the strain identified as the major cause of green mold disease in Ireland and the United Kingdom grouped with these North American isolates in having very similar randomly amplified polymorphic DNA patterns.

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^* Corresponding author. Mailing address: Department of Biological Sciences, Brock University, St. Catharines, Ontario, Canada L2S 3A1. Phone: (905) 688 5550 ext. 3598. Fax: (905) 688 1855. E-mail: acastle{at}spartan.ac.brocku.ca.

Present address: Ontario Ministry of Agriculture, Food and Rural Affairs, Ridgetown College of Agricultural Technology, Ridgetown, Ontario, Canada N0P 2C0.

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