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Appl Environ Microbiol, January 1998, p. 238-245, Vol. 64, No. 1
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Effect of Metal-Rich Sludge Amendments on the Soil Microbial Community

Erland Bååth,1,* Montserrat Díaz-Raviña,1,dagger Åsa Frostegård,1 and Colin D. Campbell2

Department of Microbial Ecology, Lund University, S-223 62 Lund, Sweden,1 and Soil Science Group, Macaulay Land Use Research Institute, Craigiebuckler, Aberdeen AB15 8QH, United Kingdom2

Received 2 July 1997/Accepted 15 October 1997

The effects of heavy-metal-containing sewage sludge on the soil microbial community were studied in two agricultural soils of different textures, which had been contaminated separately with three predominantly single metals (Cu, Zn, and Ni) at two different levels more than 20 years ago. We compared three community-based microbiological measurements, namely, phospholipid fatty acid (PLFA) analysis to reveal changes in species composition, the Biolog system to indicate metabolic fingerprints of microbial communities, and the thymidine incorporation technique to measure bacterial community tolerance. In the Luddington soil, bacterial community tolerance increased in all metal treatments compared to an unpolluted-sludge-treated control soil. Community tolerance to specific metals increased the most when the same metal was added to the soil; for example, tolerance to Cu increased most in Cu-polluted treatments. A dose-response effect was also evident. There were also indications of cotolerance to metals whose concentration had not been elevated by the sludge treatment. The PLFA pattern changed in all metal treatments, but the interpretation was complicated by the soil moisture content, which also affected the results. The Biolog measurements indicated similar effects of metals and moisture to the PLFA measurements, but due to high variation between replicates, no significant differences compared to the uncontaminated control were found. In the Lee Valley soil, significant increases in community tolerance were found for the high levels of Cu and Zn, while the PLFA pattern was significantly altered for the soils with high levels of Cu, Ni, and Zn. No effects on the Biolog measurements were found in this soil.

* Corresponding author. Mailing address: Department of Microbial Ecology, Ecology Building, Lund University, S-223 62 Lund, Sweden. Phone: (46) 46-222 42 64. Fax: (46) 46-222 41 58. E-mail: erland.baath{at}mbioekol.lu.se.

dagger Present address: Plant Biology and Soil Science Department, Vigo University, 32004 Orense, Spain.




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