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Applied and Environmental Microbiology, October 1998, p. 3584-3590, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Effects of pH and Oxygen and Ammonium Concentrations on the Community Structure of Nitrifying Bacteria from Wastewater

Alenka Princic,1,2,* Ivan Mahne,1 France Megusar,1 Eldor A. Paul,2 and James M. Tiedje2

Biotechnical Faculty, University of Ljubljana, Biology Center, 1000 Ljubljana, Slovenia,1 and Center for Microbial Ecology, Michigan State University, East Lansing, Michigan 48824-13252

Received 13 February 1998/Accepted 29 June 1998

Shifts in nitrifying community structure and function in response to different ammonium concentrations (50, 500, 1,000, and 3,000 mg of N liter-1), pH values (pH 6.0, 7.0, and 8.2), and oxygen concentrations (1, 7, and 21%) were studied in experimental reactors inoculated with nitrifying bacteria from a wastewater treatment plant. The abilities of the communities selected for these conditions to regain their original structures after conditions were returned to the original conditions were also determined. Changes in nitrifying community structure were determined by performing an amplified ribosomal DNA (rDNA) restriction analysis of PCR products obtained with ammonia oxidizer-specific rDNA primers, by phylogenetic probing, by small-subunit (SSU) rDNA sequencing, and by performing a cellular fatty acid analysis. Digestion of ammonia-oxidizer SSU rDNA with five restriction enzymes showed that a high ammonium level resulted in a great community structure change that was reversible once the ammonium concentration was returned to its original level. The smaller changes in community structure brought about by the two pH extremes, however, were irreversible. Sequence analysis revealed that the highest ammonium environment stimulated growth of a nitrifier strain that exhibited 92.6% similarity in a partial SSU rRNA sequence to its nearest relative, Nitrosomonas eutropha C-91, although the PCR product did not hybridize with a general phylogenetic probe for ammonia oxidizers belonging to the beta  subgroup of the class Proteobacteria. A principal-component analysis of fatty acid methyl ester data detected changes from the starter culture in all communities under the new selective conditions, but after the standard conditions were restored, all communities produced the original fatty acid profiles.

* Corresponding author. Present address: Netherlands Institute of Ecology, Centre for Limnology, Rijksstraatweg 6, 3631 AC Nieuwersluis, The Netherlands. Phone: 31 294 23 93 00. Fax: 31 294 23 22 24. E-mail: princic{at}cl.nioo.knaw.nl.

Applied and Environmental Microbiology, October 1998, p. 3584-3590, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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