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Applied and Environmental Microbiology, October 1998, p. 3887-3892, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

New Hybrids between Saccharomyces Sensu Stricto Yeast Species Found among Wine and Cider Production Strains

Isabelle Masneuf,1,* Jørgen Hansen,2 Casper Groth,3 Jure Piskur,3 and Denis Dubourdieu1

Faculté d'Oenologie de Bordeaux, 33400 Talence, France,1 and Carlsberg Research Laboratory, DK-2500 Copenhagen Valby,2 and Department of Microbiology, Technical University of Denmark, DTU-301, DK-2800 Lyngby,3 Denmark

Received 20 April 1998/Accepted 22 July 1998

Two yeast isolates, a wine-making yeast first identified as a Mel+ strain (ex. S. uvarum) and a cider-making yeast, were characterized for their nuclear and mitochondrial genomes. Electrophoretic karyotyping analyses, restriction fragment length polymorphism maps of PCR-amplified MET2 gene fragments, and the sequence analysis of a part of the two MET2 gene alleles found support the notion that these two strains constitute hybrids between Saccharomyces cerevisiae and Saccharomyces bayanus. The two hybrid strains had completely different restriction patterns of mitochondrial DNA as well as different sequences of the OLI1 gene. The sequence of the OLI1 gene from the wine hybrid strain appeared to be the same as that of the S. cerevisiae gene, whereas the OLI1 gene of the cider hybrid strain is equally divergent from both putative parents, S. bayanus and S. cerevisiae. Some fermentative properties were also examined, and one phenotype was found to reflect the hybrid nature of these two strains. The origin and nature of such hybridization events are discussed.

* Corresponding author. Mailing address: Faculté d'Oenologie de Bordeaux, Université Victor Segalen-Bordeaux 2, 351, Cours de la Libération, 33400 Talence, France. Phone: 33 5 56 84 64 90. Fax: 33 5 56 84 64 68. E-mail: Isabelle.Masneuf{at}oenologie.u-bordeaux2.fr.

Applied and Environmental Microbiology, October 1998, p. 3887-3892, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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