This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jalava, K.
Right arrow Articles by Hänninen, M.-L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jalava, K.
Right arrow Articles by Hänninen, M.-L.
Agricola
Right arrow Articles by Jalava, K.
Right arrow Articles by Hänninen, M.-L.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 1998, p. 3998-4006, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Isolation and Identification of Helicobacter spp. from Canine and Feline Gastric Mucosa

Katri Jalava,1,* Stephen L. W. On,2 Peter A. R. Vandamme,3,4 Irmeli Happonen,5 Antti Sukura,6 and Marja-Liisa Hänninen1

Department of Food and Environmental Hygiene,1 Department of Clinical Sciences,5 and Department of Basic Veterinary Sciences,6 Faculty of Veterinary Medicine, FIN-00014 University of Helsinki, Finland; Danish Veterinary Laboratory, Copenhagen, Denmark2; and Departments of Microbiology, University of Ghent, Ghent3 and University Hospital UIA, Antwerp,4 Belgium

Received 27 April 1998/Accepted 20 July 1998

It is known that virtually all healthy adult dogs and cats harbor spiral helicobacters in their gastric mucosa. Three species, Helicobacter felis, Helicobacter bizzozeronii, and Helicobacter salomonis have been isolated in vitro from the gastric mucosa of these animals. The aims of this study were to evaluate the efficacy of an isolation method for canine and feline gastric helicobacters that has been developed at the University of Helsinki; to estimate the prevalence and distribution of these taxa in the samples examined; and to assess the efficacy and validity of an extensive set of standardized conventional phenotypic tests, whole-cell protein profiling, and ultrastructural analysis in identifying the different species isolated from canine and feline gastric mucosa. We cultured 95 and 22 gastric mucosal biopsies from dogs and cats, respectively. Twenty-one H. bizzozeronii strains, 8 H. felis strains, 8 H. salomonis strains, 3 mixed cultures, 2 "Flexispira rappini"-like organisms, and 3 as yet uncharacterized strains were isolated from the dogs, and 3 H. felis strains were isolated from the cats. The methods used here yielded Helicobacter isolation rates of 51% from dogs and 13.6% from cats, which exceed those reported previously. The main difficulties were primary isolation, mixed cultures, and identification to the species level. In the species identification, a detailed morphological examination was found to yield important phenotypic characteristics. A large panel of biochemical and tolerance tests did not clearly differentiate the closely related species H. bizzozeronii, H. felis, and H. salomonis. Highly standardized whole-cell protein profiling was shown to be an excellent method for species identification. Improvements in culture conditions for these bacteria are still needed, especially for cats. A genetic identification method not requiring culture is needed for future studies of these very fastidious helicobacters, as the clinical significance and ecology of these species within the gastric mucosa of the domestic carnivores remain largely unknown.

* Corresponding author. Mailing address: Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, P.O. Box 57, FIN-00014 University of Helsinki, Finland. Phone: 358-9-70849705. Fax: 358-9-70849718. E-mail: Katri.Jalava{at}Helsinki.Fi.

Applied and Environmental Microbiology, October 1998, p. 3998-4006, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pregel, P., Rota, A., Palmerini, D., Guarda, F., Appino, S. (2008). Detection of Helicobacter in gastric washing of cats. jvdi 20: 780-782 [Abstract] [Full Text]  
  • Baele, M., Decostere, A., Vandamme, P., Ceelen, L., Hellemans, A., Mast, J., Chiers, K., Ducatelle, R., Haesebrouck, F. (2008). Isolation and characterization of Helicobacter suis sp. nov. from pig stomachs. Int. J. Syst. Evol. Microbiol. 58: 1350-1358 [Abstract] [Full Text]  
  • Baele, M., Decostere, A., Vandamme, P., Van den Bulck, K., Gruntar, I., Mehle, J., Mast, J., Ducatelle, R., Haesebrouck, F. (2008). Helicobacter baculiformis sp. nov., isolated from feline stomach mucosa. Int. J. Syst. Evol. Microbiol. 58: 357-364 [Abstract] [Full Text]  
  • Hannula, M., Hanninen, M.-L. (2007). Phylogenetic analysis of Helicobacter species based on partial gyrB gene sequences. Int. J. Syst. Evol. Microbiol. 57: 444-449 [Abstract] [Full Text]  
  • Van den Bulck, K., Decostere, A., Baele, M., Vandamme, P., Mast, J., Ducatelle, R., Haesebrouck, F. (2006). Helicobacter cynogastricus sp. nov., isolated from the canine gastric mucosa.. Int. J. Syst. Evol. Microbiol. 56: 1559-1564 [Abstract] [Full Text]  
  • Van den Bulck, K., Decostere, A., Gruntar, I., Baele, M., Krt, B., Ducatelle, R., Haesebrouck, F. (2005). In Vitro Antimicrobial Susceptibility Testing of Helicobacter felis, H. bizzozeronii, and H. salomonis. Antimicrob. Agents Chemother. 49: 2997-3000 [Abstract] [Full Text]  
  • Van den Bulck, K., Decostere, A., Baele, M., Driessen, A., Debongnie, J.-C., Burette, A., Stolte, M., Ducatelle, R., Haesebrouck, F. (2005). Identification of Non-Helicobacter pylori Spiral Organisms in Gastric Samples from Humans, Dogs, and Cats. J. Clin. Microbiol. 43: 2256-2260 [Abstract] [Full Text]  
  • Hanninen, M.-L., Karenlampi, R. I., Koort, J. M. K., Mikkonen, T., Bjorkroth, K. J. (2005). Extension of the species Helicobacter bilis to include the reference strains of Helicobacter sp. flexispira taxa 2, 3 and 8 and Finnish canine and feline flexispira strains. Int. J. Syst. Evol. Microbiol. 55: 891-898 [Abstract] [Full Text]  
  • Mikkonen, T. P., Karenlampi, R. I., Hanninen, M.-L. (2004). Phylogenetic analysis of gastric and enterohepatic Helicobacter species based on partial HSP60 gene sequences. Int. J. Syst. Evol. Microbiol. 54: 753-758 [Abstract] [Full Text]  
  • Baele, M., Van den Bulck, K., Decostere, A., Vandamme, P., Hanninen, M.-L., Ducatelle, R., Haesebrouck, F. (2004). Multiplex PCR Assay for Differentiation of Helicobacter felis, H. bizzozeronii, and H. salomonis. J. Clin. Microbiol. 42: 1115-1122 [Abstract] [Full Text]  
  • Al-Soud, W. A., Bennedsen, M., On, S. L. W., Ouis, I.-S., Vandamme, P., Nilsson, H.-O., Ljungh, A., Wadstrom, T. (2003). Assessment of PCR-DGGE for the identification of diverse Helicobacter species, and application to faecal samples from zoo animals to determine Helicobacter prevalence. J Med Microbiol 52: 765-771 [Abstract] [Full Text]  
  • Hanninen, M.-L., Utriainen, M., Happonen, I., Dewhirst, F. E. (2003). Helicobacter sp. flexispira 16S rDNA taxa 1, 4 and 5 and Finnish porcine Helicobacter isolates are members of the species Helicobacter trogontum (taxon 6). Int. J. Syst. Evol. Microbiol. 53: 425-433 [Abstract] [Full Text]  
  • Kostia, S., Veijalainen, P., Hirvi, U., Hanninen, M.-L. (2003). Cytolethal distending toxin B gene (cdtB) homologues in taxa 2, 3 and 8 and in six canine isolates of Helicobacter sp. flexispira. J Med Microbiol 52: 103-108 [Abstract] [Full Text]  
  • De Groote, D., Haesebrouck, F., van Doorn, L.-J., Vandamme, P., Ducatelle, R. (2001). Evaluation of a Group-Specific 16S Ribosomal DNA-Based PCR for Detection of Helicobacter bizzozeronii, Helicobacter felis, and Helicobacter salomonis in Fresh and Paraffin-Embedded Gastric Biopsy Specimens. J. Clin. Microbiol. 39: 1197-1199 [Abstract] [Full Text]  
  • Solnick, J. V., Schauer, D. B. (2001). Emergence of Diverse Helicobacter Species in the Pathogenesis of Gastric and Enterohepatic Diseases. Clin. Microbiol. Rev. 14: 59-97 [Abstract] [Full Text]  
  • Vandamme, P., Harrington, C. S., Jalava, K., On, S. L. W. (2000). Misidentifying Helicobacters: the Helicobacter cinaedi Example. J. Clin. Microbiol. 38: 2261-2266 [Abstract] [Full Text]  
  • Simpson, K. W., Strauss-Ayali, D., Scanziani, E., Straubinger, R. K., McDonough, P. L., Straubinger, A. F., Chang, Y.-F., Domeneghini, C., Arebi, N., Calam, J. (2000). Helicobacter felis Infection Is Associated with Lymphoid Follicular Hyperplasia and Mild Gastritis but Normal Gastric Secretory Function in Cats. Infect. Immun. 68: 779-790 [Abstract] [Full Text]  
  • Sorlin, P., Vandamme, P., Nortier, J., Hoste, B., Rossi, C., Pavlof, S., Struelens, M. J. (1999). Recurrent "Flexispira rappini" Bacteremia in an Adult Patient Undergoing Hemodialysis: Case Report. J. Clin. Microbiol. 37: 1319-1323 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»