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Applied and Environmental Microbiology, October 1998, p. 4028-4034, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification, Characterization, and In Situ Detection of a Fruit-Body-Specific Hydrophobin of Pleurotus ostreatus

María M. Peñas,1 Sigridur A. Ásgeirsdóttir,2 Iñigo Lasa,1 Francisco A. Culiañez-Macià,3 Antonio G. Pisabarro,1 Joseph G. H. Wessels,2 and Lucía Ramírez1,*

Departamento de Producción Agraria, Universidad Pública de Navarra, E-31006 Pamplona,1 and Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas, Universidad Politécnica de Valencia, E-46022 Valencia,3 Spain, and Department of Plant Biology, University of Groningen, 9751 NN Haren, The Netherlands2

Received 20 May 1998/Accepted 30 June 1998

Hydrophobins are small (length, about 100 ± 25 amino acids), cysteine-rich, hydrophobic proteins that are present in large amounts in fungal cell walls, where they form part of the outermost layer (rodlet layer); sometimes, they can also be secreted into the medium. Different hydrophobins are associated with different developmental stages of a fungus, and their biological functions include protection of the hyphae against desiccation and attack by either bacterial or fungal parasites, hyphal adherence, and the lowering of surface tension of the culture medium to permit aerial growth of the hyphae. We identified and isolated a hydrophobin (fruit body hydrophobin 1 [Fbh1]) present in fruit bodies but absent in both monokaryotic and dikaryotic mycelia of the edible mushroom Pleurotus ostreatus. In order to study the temporal and spatial expression of the fbh1 gene, we determined the N-terminal amino acid sequence of Fbh1. We also synthesized and cloned the double-stranded cDNA corresponding to the full-length mRNA of Fbh1 to use it as a probe in both Northern blot and in situ hybridization experiments. Fbh1 mRNA is detectable in specific parts of the fruit body, and it is absent in other developmental stages.

* Corresponding author. Mailing address: Departamento de Producción Agraria, Universidad Pública de Navarra, E-31006 Pamplona, Spain. Phone: (34) 948 169 130. Fax: (34) 948 169 732. E-mail: Iramirez{at}upna.es.

Applied and Environmental Microbiology, October 1998, p. 4028-4034, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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