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Applied and Environmental Microbiology, October 1998, p. 4076-4078, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Balance of Activities of Alcohol Acetyltransferase and Esterase in Saccharomyces cerevisiae Is Important for Production of Isoamyl Acetate

Kiyoshi Fukuda,1,* Nagi Yamamoto,1 Yoshifumi Kiyokawa,1 Toshiyasu Yanagiuchi,1 Yoshinori Wakai,1 Katsuhiko Kitamoto,2 Yoshiharu Inoue,3 and Akira Kimura3

Kizakura Sake Brewing Co., Ltd., Fushimi-ku, Kyoto 612-8046,1 Department of Biotechnology, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032,2 and Research Institute for Food Science, Kyoto University, Uji, Kyoto 611-0011,3 Japan

Received 17 February 1998/Accepted 21 July 1998

Isoamyl acetate is synthesized from isoamyl alcohol and acetyl coenzyme A by alcohol acetyltransferase (AATFase) in Saccharomyces cerevisiae and is hydrolyzed by esterases at the same time. We hypothesized that the balance of both enzyme activities was important for optimum production of isoamyl acetate in sake brewing. To test this hypothesis, we constructed yeast strains with different numbers of copies of the AATFase gene (ATF1) and the isoamyl acetate-hydrolyzing esterase gene (IAH1) and used these strains in small-scale sake brewing. Fermentation profiles as well as components of the resulting sake were largely alike; however, the amount of isoamyl acetate in the sake increased with an increasing ratio of AATFase/Iah1p esterase activity. Therefore, we conclude that the balance of these two enzyme activities is important for isoamyl acetate accumulation in sake mash.

* Corresponding author. Mailing address: Kizakura Sake Brewing Co., Ltd., 223 Shioya-machi, Fushimi-ku, Kyoto 612-8046, Japan. Phone: 81 75 611 4101, ext. 351. Fax: 81 75 622 3510. E-mail: kizakura{at}mbox.kyoto-inet.or.jp.

Applied and Environmental Microbiology, October 1998, p. 4076-4078, Vol. 64, No. 10
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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