This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Adams, C. A.
Right arrow Articles by McIntosh, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Adams, C. A.
Right arrow Articles by McIntosh, D.
Agricola
Right arrow Articles by Adams, C. A.
Right arrow Articles by McIntosh, D.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 1998, p. 4194-4201, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Characterization of Plasmid-Mediated Oxytetracycline Resistance in Aeromonas salmonicida

C. A. Adams, B. Austin,* P. G. Meaden, and D. McIntoshdagger

Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh, EH14 4AS, Scotland

Received 20 March 1998/Accepted 13 August 1998

Using broth conjugation, we found that 19 of 29 (66%) oxytetracycline (OT)-resistant isolates of Aeromonas salmonicida transferred the OT resistance phenotype to Escherichia coli. The OT resistance phenotype was encoded by high-molecular-weight R-plasmids that were capable of transferring OT resistance to both environmental and clinical isolates of Aeromonas spp. The molecular basis for antibiotic resistance in OT-resistant isolates of A. salmonicida was determined. The OT resistance determinant from one plasmid (pASOT) of A. salmonicida was cloned and used in Southern blotting and hybridization experiments as a probe. The determinant was identified on a 5.4-kb EcoRI fragment on R-plasmids from the 19 OT-resistant isolates of A. salmonicida. Hybridization with plasmids encoding the five classes (classes A to E) of OT resistance determinants demonstrated that the OT resistance plasmids of the 19 A. salmonicida isolates carried the class A resistance determinant. Analysis of data generated from restriction enzyme digests showed that the OT resistance plasmids were not identical; three profiles were characterized, two of which showed a high degree of homology.

* Corresponding author. Mailing address: Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh, EH14 4AS, United Kingdom. Phone: 44-131-451-3452. Fax: 44-131-451-3009. E-mail: B.Austin{at}hw.ac.uk.

dagger Present address: Departamento de Bioquimica e Biologia Molecular, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, 21045-900, Brazil.

Applied and Environmental Microbiology, November 1998, p. 4194-4201, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Dhanarani, T. S., Shankar, C., Park, J., Dexilin, M., Kumar, R. R., Thamaraiselvi, K. (2009). Study on acquisition of bacterial antibiotic resistance determinants in poultry litter. Poult. Sci. 88: 1381-1387 [Abstract] [Full Text]  
  • Kulinska, A., Czeredys, M., Hayes, F., Jagura-Burdzy, G. (2008). Genomic and Functional Characterization of the Modular Broad-Host-Range RA3 Plasmid, the Archetype of the IncU Group. Appl. Environ. Microbiol. 74: 4119-4132 [Abstract] [Full Text]  
  • McIntosh, D., Cunningham, M., Ji, B., Fekete, F. A., Parry, E. M., Clark, S. E., Zalinger, Z. B., Gilg, I. C., Danner, G. R., Johnson, K. A., Beattie, M., Ritchie, R. (2008). Transferable, multiple antibiotic and mercury resistance in Atlantic Canadian isolates of Aeromonas salmonicida subsp. salmonicida is associated with carriage of an IncA/C plasmid similar to the Salmonella enterica plasmid pSN254. J Antimicrob Chemother 61: 1221-1228 [Abstract] [Full Text]  
  • Nawaz, M., Sung, K., Khan, S. A., Khan, A. A., Steele, R. (2006). Biochemical and Molecular Characterization of Tetracycline-Resistant Aeromonas veronii Isolates from Catfish.. Appl. Environ. Microbiol. 72: 6461-6466 [Abstract] [Full Text]  
  • Rhodes, G., Parkhill, J., Bird, C., Ambrose, K., Jones, M. C., Huys, G., Swings, J., Pickup, R. W. (2004). Complete Nucleotide Sequence of the Conjugative Tetracycline Resistance Plasmid pFBAOT6, a Member of a Group of IncU Plasmids with Global Ubiquity. Appl. Environ. Microbiol. 70: 7497-7510 [Abstract] [Full Text]  
  • Neuendorf, S., Hedtke, K., Tangen, G., Genersch, E. (2004). Biochemical characterization of different genotypes of Paenibacillus larvae subsp. larvae, a honey bee bacterial pathogen. Microbiology 150: 2381-2390 [Abstract] [Full Text]  
  • Furushita, M., Shiba, T., Maeda, T., Yahata, M., Kaneoka, A., Takahashi, Y., Torii, K., Hasegawa, T., Ohta, M. (2003). Similarity of Tetracycline Resistance Genes Isolated from Fish Farm Bacteria to Those from Clinical Isolates. Appl. Environ. Microbiol. 69: 5336-5342 [Abstract] [Full Text]  
  • Sorum, H., L'Abee-Lund, T. M., Solberg, A., Wold, A. (2003). Integron-Containing IncU R Plasmids pRAS1 and pAr-32 from the Fish Pathogen Aeromonas salmonicida. Antimicrob. Agents Chemother. 47: 1285-1290 [Abstract] [Full Text]  
  • Nonaka, L., Suzuki, S. (2002). New Mg2+-Dependent Oxytetracycline Resistance Determinant Tet 34 in Vibrio Isolates from Marine Fish Intestinal Contents. Antimicrob. Agents Chemother. 46: 1550-1552 [Abstract] [Full Text]  
  • Schmidt, A. S., Bruun, M. S., Dalsgaard, I., Larsen, J. L. (2001). Incidence, Distribution, and Spread of Tetracycline Resistance Determinants and Integron-Associated Antibiotic Resistance Genes among Motile Aeromonads from a Fish Farming Environment. Appl. Environ. Microbiol. 67: 5675-5682 [Abstract] [Full Text]  
  • Schmidt, A. S., Bruun, M. S., Larsen, J. L., Dalsgaard, I. (2001). Characterization of class 1 integrons associated with R-plasmids in clinical Aeromonas salmonicida isolates from various geographical areas. J Antimicrob Chemother 47: 735-743 [Abstract] [Full Text]  
  • Rhodes, G., Huys, G., Swings, J., Mcgann, P., Hiney, M., Smith, P., Pickup, R. W. (2000). Distribution of Oxytetracycline Resistance Plasmids between Aeromonads in Hospital and Aquaculture Environments: Implication of Tn1721 in Dissemination of the Tetracycline Resistance Determinant Tet A. Appl. Environ. Microbiol. 66: 3883-3890 [Abstract] [Full Text]  
  • Goñi-Urriza, M., Capdepuy, M., Arpin, C., Raymond, N., Caumette, P., Quentin, C. (2000). Impact of an Urban Effluent on Antibiotic Resistance of Riverine Enterobacteriaceae and Aeromonas spp.. Appl. Environ. Microbiol. 66: 125-132 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»