This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Denis-Larose, C.
Right arrow Articles by Lau, P. C. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Denis-Larose, C.
Right arrow Articles by Lau, P. C. K.
Agricola
Right arrow Articles by Denis-Larose, C.
Right arrow Articles by Lau, P. C. K.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 1998, p. 4363-4367, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of the Basic Replicon of Rhodococcus Plasmid pSOX and Development of a Rhodococcus-Escherichia coli Shuttle Vectordagger

Claude Denis-Larose,1 Hélène Bergeron,1 Diane Labbé,1 Charles W. Greer,1 Jalal Hawari,1 Matthew J. Grossman,2 Bruce M. Sankey,3 and Peter C. K. Lau1,*

Biotechnology Research Institute, National Research Council Canada, Montreal, Quebec, Canada H4P 2R21; Exxon Research and Engineering Company, Corporate Research, Annandale, New Jersey 088012; and Imperial Oil Resources Ltd., Calgary, Alberta, Canada T2L 2K83

Received 14 May 1998/Accepted 24 August 1998

The replication region of a 100-kb desulfurization plasmid (pSOX) from Rhodococcus sp. strain X309 was localized to a 4-kb KpnI fragment, and its sequence was determined. The amino acid sequence of one of the predicted open reading frames (ORFs) was related to the putative replication (Rep) protein sequences of the mycobacterial pLR7 family of plasmids. Three of the five predicted ORF products were identified by radiolabelling with the Escherichia coli T7 polymerase/promoter system. In E. coli, the Rep protein of pSOX was apparently synthesized in a shortened form, 21.3 kDa instead of the predicted 41.3 kDa, as a result of an internal initiation. This situation is reminescent of that for some bacterial Rep proteins. A shuttle plasmid was constructed with the pSOX origin, pBluescript II KS-, and the chloramphenicol resistance (Cmr) gene from pRF29. This new shuttle plasmid was used to demonstrate expression of the Bacillus subtilis sacB gene in a strain of Rhodococcus, rendering it sensitive to the presence of sucrose.

* Corresponding author. Mailing address: Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Ave., Montreal, Quebec, Canada H4P 2R2. Phone: (514) 496 6325. Fax: (514) 496 6265. E-mail: peter.lau{at}nrc.ca.

dagger NRCC publication 41774.

Applied and Environmental Microbiology, November 1998, p. 4363-4367, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Stinear, T. P., Pryor, M. J., Porter, J. L., Cole, S. T. (2005). Functional analysis and annotation of the virulence plasmid pMUM001 from Mycobacterium ulcerans. Microbiology 151: 683-692 [Abstract] [Full Text]  
  • Warren, R., Hsiao, W. W. L., Kudo, H., Myhre, M., Dosanjh, M., Petrescu, A., Kobayashi, H., Shimizu, S., Miyauchi, K., Masai, E., Yang, G., Stott, J. M., Schein, J. E., Shin, H., Khattra, J., Smailus, D., Butterfield, Y. S., Siddiqui, A., Holt, R., Marra, M. A., Jones, S. J. M., Mohn, W. W., Brinkman, F. S. L., Fukuda, M., Davies, J., Eltis, L. D. (2004). Functional Characterization of a Catabolic Plasmid from Polychlorinated- Biphenyl-Degrading Rhodococcus sp. Strain RHA1. J. Bacteriol. 186: 7783-7795 [Abstract] [Full Text]  
  • Hosted, T. J., Wang, T., Horan, A. C. (2004). Characterization of the Streptomyces lavendulae IMRU 3455 linear plasmid pSLV45. Microbiology 150: 1819-1827 [Abstract] [Full Text]  
  • Broker, D., Arenskotter, M., Legatzki, A., Nies, D. H., Steinbuchel, A. (2004). Characterization of the 101-Kilobase-Pair Megaplasmid pKB1, Isolated from the Rubber-Degrading Bacterium Gordonia westfalica Kb1. J. Bacteriol. 186: 212-225 [Abstract] [Full Text]  
  • Takai, S., Hines, S. A., Sekizaki, T., Nicholson, V. M., Alperin, D. A., Osaki, M., Takamatsu, D., Nakamura, M., Suzuki, K., Ogino, N., Kakuda, T., Dan, H., Prescott, J. F. (2000). DNA Sequence and Comparison of Virulence Plasmids from Rhodococcus equi ATCC 33701 and 103. Infect. Immun. 68: 6840-6847 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»