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Applied and Environmental Microbiology, November 1998, p. 4588-4590, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

In Situ Detection of an Uncultured Predominant Bacillus in Dutch Grassland Soils

Andreas Felske,* Antoon D. L. Akkermans, and Willem M. De Vos

Laboratory of Microbiology, Department of Biomolecular Sciences, Wageningen Agricultural University, 6703 CT Wageningen, The Netherlands

Received 20 April 1998/Accepted 7 July 1998

Uncultured predominant Bacillus ribotype DA001 in Dutch Drentse A grassland soils, as revealed by its 16S rRNA sequence, was detected in soil by fluorescent whole-cell in situ hybridization. A prominent rod-shaped cell type was identified in bacterial suspensions prepared from soil by a multiple 16S rRNA probing approach.

* Corresponding author. Present address: Instituto de Recursos Naturales y Agrobiologia, C.S.I.C., Apartado 1052, 41080 Seville, Spain. Phone: 34 462 4711, ext. 131. Fax: 34 462 4002. E-mail: Andreas{at}cica.es.

Applied and Environmental Microbiology, November 1998, p. 4588-4590, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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