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Applied and Environmental Microbiology, November 1998, p. 4614-4617, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evidence for the Interplay between Trehalose Metabolism and Hsp104 in Yeast

Hitoshi Iwahashi,* Solomon Nwaka, Kaoru Obuchi, and Yasuhiko Komatsu

National Institute of Bioscience and Human-Technology, Tsukuba, Ibaraki 305-8566, Japan

Received 29 June 1998/Accepted 7 September 1998

Disruption of the HSP104 gene in a mutant which cannot accumulate trehalose during heat shock treatment caused trehalose accumulation (H. Iwahashi, K. Obuchi, S. Fujii, and Y. Komatsu, Lett. Appl. Microbiol 25:43-47, 1997). This implies that Hsp104 affects trehalose metabolism. Thus, we measured the activities of enzymes involved in trehalose metabolism. The activities of trehalose-synthesizing and -hydrolyzing enzymes are low in the HSP104 disruption mutant during heat shock. This data is correlated with intracellular trehalose and glucose levels observed in the HSP104 disruption mutant. These results suggest that during heat shock, Hsp104 contributes to the simultaneous increase in both accumulation and degradation of trehalose.

* Corresponding author. Mailing address: National Institute of Bioscience and Human-Technology, Higashi 1-1, Tsukuba, Ibaraki 305-8566, Japan. Phone: 81-298-54-6059. Fax: 81-298-54-6009. E-mail: iwahashi{at}nibh.go.jp.

Applied and Environmental Microbiology, November 1998, p. 4614-4617, Vol. 64, No. 11
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pacheco, A., Pereira, C., Almeida, M. J., Sousa, M. J. (2009). Small heat-shock protein Hsp12 contributes to yeast tolerance to freezing stress. Microbiology 155: 2021-2028 [Abstract] [Full Text]  
  • Iwahashi, H., Nwaka, S., Obuchi, K. (2000). Evidence for Contribution of Neutral Trehalase in Barotolerance of Saccharomyces cerevisiae. Appl. Environ. Microbiol. 66: 5182-5185 [Abstract] [Full Text]  


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