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Applied and Environmental Microbiology, December 1998, p. 4816-4819, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Strain-Specific Identification of Probiotic Lactobacillus rhamnosus with Randomly Amplified Polymorphic DNA-Derived PCR Primers

Anu Tilsala-Timisjärvi1,* and Tapani Alatossava1,2

Department of Biology, University of Oulu, Linnanmaa, FIN-90570 Oulu,1 and Biotechnology Laboratory, REDEC of Kajaani, University of Oulu, FIN-88600 Sotkamo,2 Finland

Received 15 June 1998/Accepted 3 September 1998

In the present work, strain-specific PCR primers for Lactobacillus rhamnosus Lc 1/3 are described. The randomly amplified polymorphic DNA (RAPD) technique was used to produce potential strain-specific markers. They were screened for specificity by hybridization with DNA from 11 L. rhamnosus strains. A 613-bp RAPD marker found to be strain-specific was sequenced, and a primer pair specific to L. rhamnosus Lc 1/3 was constructed based on the sequence. The primer pair was tested with 11 Lactobacillus species and 11 L. rhamnosus strains and was found to be strain specific. The nucleotide sequence of the specific RAPD marker was found to contain part of a protein encoding region which showed significant similarity to several transposases for insertion sequence elements of various bacteria, including other lactic acid bacterium species.

* Corresponding author. Mailing address: Department of Biology, University of Oulu, Linnanmaa, FIN-90570 Oulu, Finland. Phone: 358 8 553 1792. Fax: 358 8 553 1061. E-mail: atilsala{at}cc.oulu.fi.

Applied and Environmental Microbiology, December 1998, p. 4816-4819, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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