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Applied and Environmental Microbiology, December 1998, p. 4912-4917, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Genetic Diversity and Phylogeny of Rhizobia That Nodulate Acacia spp. in Morocco Assessed by Analysis of rRNA Genes

Bouchaib Khbaya,1 Marc Neyra,2 Philippe Normand,3 Karim Zerhari,1 and Abdelkarim Filali-Maltouf1,*

Laboratoire de Microbiologie et Biologie Moléculaire, Faculté des Sciences, Université Mohammed V, Rabat, Morocco1; Laboratoire d'Écologie Microbienne des Sols, ORSTOM-Dakar, Sénégal2; and Laboratoire de Microbiologie des Sols, UMR CNRS 5557, Université Claude-Bernard, Lyon 1, 69622 Villeurbanne Cedex, France3

Received 15 May 1998/Accepted 24 August 1998

Forty rhizobia nodulating four Acacia species (A. gummifera, A. raddiana, A. cyanophylla, and A. horrida) were isolated from different sites in Morocco. These rhizobia were compared by analyzing both the 16S rRNA gene (rDNA) and the 16S-23S rRNA spacer by PCR with restriction fragment length polymorphism (RFLP) analysis. Analysis of the length of 16S-23S spacer showed a considerable diversity within these microsymbionts, but RFLP analysis of the amplified spacer revealed no additional heterogeneity. Three clusters were identified when 16S rDNA analysis was carried out. Two of these clusters include some isolates which nodulate, nonspecifically, the four Acacia species. These clusters, A and B, fit within the Sinorhizobium lineage and are closely related to S. meliloti and S. fredii, respectively. The third cluster appeared to belong to the Agrobacterium-Rhizobium galegae phylum and is more closely related to the Agrobacterium tumefaciens species. These relations were confirmed by sequencing a representative strain from each cluster.

* Corresponding author. Mailing address: Laboratoire de Microbiologie et Biologie Moléculaire, Faculté des Sciences, Université Mohammed V, Rabat, Morocco. Phone and fax: (212) 7 77 54 61. E-mail: filali{at}fsr.ac.ma.

Applied and Environmental Microbiology, December 1998, p. 4912-4917, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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