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Applied and Environmental Microbiology, December 1998, p. 4944-4949, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection and Isolation of Novel Rhizopine-Catabolizing Bacteria from the Environment

Brian B. McSpadden Gardener1,2,3 and Frans J. de Bruijn2,3,4,*

Department of Botany and Plant Pathology,1 MSU-DOE Plant Research Laboratory,2 Department of Microbiology,4 and NSF Center for Microbial Ecology,3 Michigan State University, East Lansing, Michigan 48824

Received 1 June 1998/Accepted 27 September 1998

Microbial rhizopine-catabolizing (Moc) activity was detected in serial dilutions of soil and rhizosphere washes. The activity observed generally ranged between 106 and 107 catabolic units per g, and the numbers of nonspecific culture-forming units were found to be approximately 10 times higher. A diverse set of 37 isolates was obtained by enrichment on scyllo-inosamine-containing media. However, none of the bacteria that were isolated were found to contain DNA sequences homologous to the known mocA, mocB, and mocC genes of Sinorhizobium meliloti L5-30. Twenty-one of the isolates could utilize an SI preparation as the sole carbon and nitrogen source for growth. Partial sequencing of 16S ribosomal DNAs (rDNAs) amplified from these strains indicated that five distinct bacterial genera (Arthrobacter, Sinorhizobium, Pseudomonas, Aeromonas, and Alcaligenes) were represented in this set. Only 6 of these 21 isolates could catabolize 3-O-methyl-scyllo-inosamine under standard assay conditions. Two of these, strains D1 and R3, were found to have 16S rDNA sequences very similar to those of Sinorhizobium meliloti. However, these strains are not symbiotically effective on Medicago sativa, and DNA sequences homologous to the nodB and nodC genes were not detected in strains D1 and R3 by Southern hybridization analysis.

* Corresponding author. Mailing address: MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824. Phone: (517) 353-2229. Fax: (517) 355-9614. E-mail: debruijn{at}pilot.msu.edu.

Applied and Environmental Microbiology, December 1998, p. 4944-4949, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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