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Applied and Environmental Microbiology, December 1998, p. 5023-5026, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Construction of a Bioluminescent Reporter Strain To Detect Polychlorinated Biphenyls

A. C. Layton,1,* M. Muccini,1 M. M. Ghosh,2 and G. S. Sayler1

The Center for Environmental Biotechnology1 and Department of Civil and Environmental Engineering,2 The University of Tennessee, Knoxville, Tennessee 37996-1605

Received 11 June 1998/Accepted 25 September 1998

A bioluminescent reporter strain, Ralstonia eutropha ENV307(pUTK60), was constructed for the detection of polychlorinated biphenyls by inserting the biphenyl promoter upstream of the bioluminescence genes. In the presence of a nonionic surfactant, which enhances the solubility of chlorinated biphenyls, bioluminescence was induced three- to fourfold over background by biphenyl, monochlorinated biphenyls, and Aroclor 1242. The minimum detection limits for these compounds ranged from 0.15 mg/liter for 4-chlorobiphenyl to 1.5 mg/liter for Aroclor 1242.

* Corresponding author. Mailing address: The Center for Environmental Biotechnology, 676 Dabney Hall, The University of Tennessee, Knoxville, TN 37996-1605. Phone: (423) 974-8080. Fax: (423) 974-8086. E-mail: alayton{at}utk.edu.

Applied and Environmental Microbiology, December 1998, p. 5023-5026, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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