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Applied and Environmental Microbiology, December 1998, p. 5039-5041, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Simple and Reliable Method for Hybridization of Homothallic Wine Strains of Saccharomyces cerevisiae

Manuel Ramírez,1,* Francisco Peréz,1 and José A. Regodón2

Departamento de Microbiología, Universidad de Extremadura, 06071 Badajoz,1 and Instituto de Tecnología Agroalimentaria, Junta de Extremadura, 06080 Badajoz,2 Spain

Received 11 June 1998/Accepted 15 September 1998

A procedure was developed for the hybridization and improvement of homothallic industrial wine yeasts. Killer cycloheximide-sensitive strains were crossed with killer-sensitive cycloheximide-resistant strains to get killer cycloheximide-resistant hybrids, thereby enabling hybrid selection and identification. This procedure also allows backcrossing of spore colonies from the hybrids with parental strains.

* Corresponding author. Mailing address: Departamento de Microbiología, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain. Phone: 34-924-289426. Fax: 34-924-271304. E-mail: mramirez{at}unex.es.

Applied and Environmental Microbiology, December 1998, p. 5039-5041, Vol. 64, No. 12
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ramirez, M., Ambrona, J. (2008). Construction of Sterile ime1{Delta}-Transgenic Saccharomyces cerevisiae Wine Yeasts Unable To Disseminate in Nature. Appl. Environ. Microbiol. 74: 2129-2134 [Abstract] [Full Text]  
  • Ramirez, M., Vinagre, A., Ambrona, J., Molina, F., Maqueda, M., Rebollo, J. E. (2004). Genetic Instability of Heterozygous, Hybrid, Natural Wine Yeasts. Appl. Environ. Microbiol. 70: 4686-4691 [Abstract] [Full Text]  


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