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Appl Environ Microbiol, February 1998, p. 535-542, Vol. 64, No. 2
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of Marine Temperate Phage-Host Systems Isolated from Mamala Bay, Oahu, Hawaii

Sunny C. Jiang,dagger Christina A. Kellogg, and John H. Paul*

Department of Marine Science, University of South Florida, St. Petersburg, Florida 33701

Received 24 April 1997/Accepted 14 November 1997

To understand the ecological and genetic role of viruses in the marine environment, it is critical to know the infectivity of viruses and the types of interactions that occur between marine viruses and their hosts. We isolated four marine phages from turbid plaques by using four indigenous bacterial hosts obtained from concentrated water samples from Mamala Bay, Oahu, Hawaii. Two of the rod-shaped bacterial hosts were identified as Sphingomonas paucimobilis and Flavobacterium sp. All of the phage isolates were tailed phages and contained double-stranded DNA. Two of the phage isolates had morphologies typical of the family Siphoviridae, while the other two belonged to the families Myoviridae and Podoviridae. The head diameters of these viruses ranged from 47 to 70.7 nm, and the tail lengths ranged from 12 to 146 nm. The burst sizes ranged from 7.8 to 240 phage/bacterial cell, and the genome sizes, as determined by restriction digestion, ranged from 36 to 112 kb. The members of the Siphoviridae, T-phi HSIC, and T-phi D0, and the member of the Myoviridae, T-phi D1B, were found to form lysogenic associations with their bacterial hosts, which were isolated from the same water samples. Hybridization of phage T-phi HSIC probe with lysogenic host genomic DNA was observed in dot blot hybridization experiments, indicating that prophage T-phi HSIC was integrated within the host genome. These phage-host systems are available for use in studies of marine lysogeny and transduction.

* Corresponding author. Mailing address: Department of Marine Science, University of South Florida, 140 7th Avenue South, St. Petersburg, FL 33701. Phone: (813) 553-1130. Fax: (813) 553-1189. E-mail: jpaul{at}seas.marine.usf.edu.

dagger Present address: The Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, MD 21202.




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