This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marchesi, J. R.
Right arrow Articles by Wade, W. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marchesi, J. R.
Right arrow Articles by Wade, W. G.
Agricola
Right arrow Articles by Marchesi, J. R.
Right arrow Articles by Wade, W. G.

 Previous Article  |  Next Article 

Appl Environ Microbiol, February 1998, p. 795-799, Vol. 64, No. 2
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA

Julian R. Marchesi,1,* Takuichi Sato,2 Andrew J. Weightman,1 Tracey A. Martin,1 John C. Fry,1 Sarah J. Hiom,3 and William G. Wade4

School of Pure and Applied Biology, University of Wales College of Cardiff, Cardiff CF1 3TL,1 Department of Oral Medicine, Pathology and Microbiology, Centre for the Study of Oral Disease, Bristol Dental Hospital and School, Bristol BS1 2LY,3 and Department of Oral Medicine and Pathology, Guys and St. Thomas's Medical and Dental School, Guy's Hospital, London SE1 9RT,4 United Kingdom, and Department of Oral Microbiology, Niigata University School of Dentistry, Niigata 951, Japan2

Received 8 August 1997/Accepted 14 November 1997

We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a variety of bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.

* Corresponding author. Mailing address: School of Pure and Applied Biology, University of Wales College of Cardiff, P.O. Box 915, Cardiff CF1 3TL, United Kingdom. Phone: 44-1222-874000, ext. 5073. Fax: 44-1222-874305. E-mail: marchesi{at}cf.ac.uk.




This article has been cited by other articles:

  • Rooney, A. P., Price, N. P. J., Ehrhardt, C., Swezey, J. L., Bannan, J. D. (2009). Phylogeny and molecular taxonomy of the Bacillus subtilis species complex and description of Bacillus subtilis subsp. inaquosorum subsp. nov.. Int. J. Syst. Evol. Microbiol. 59: 2429-2436 [Abstract] [Full Text]  
  • Youssef, N., Sheik, C. S., Krumholz, L. R., Najar, F. Z., Roe, B. A., Elshahed, M. S. (2009). Comparison of Species Richness Estimates Obtained Using Nearly Complete Fragments and Simulated Pyrosequencing-Generated Fragments in 16S rRNA Gene-Based Environmental Surveys. Appl. Environ. Microbiol. 75: 5227-5236 [Abstract] [Full Text]  
  • Eilmus, S., Heil, M. (2009). Bacterial Associates of Arboreal Ants and Their Putative Functions in an Obligate Ant-Plant Mutualism. Appl. Environ. Microbiol. 75: 4324-4332 [Abstract] [Full Text]  
  • Raina, J.-B., Tapiolas, D., Willis, B. L., Bourne, D. G. (2009). Coral-Associated Bacteria and Their Role in the Biogeochemical Cycling of Sulfur. Appl. Environ. Microbiol. 75: 3492-3501 [Abstract] [Full Text]  
  • Kounatidis, I., Crotti, E., Sapountzis, P., Sacchi, L., Rizzi, A., Chouaia, B., Bandi, C., Alma, A., Daffonchio, D., Mavragani-Tsipidou, P., Bourtzis, K. (2009). Acetobacter tropicalis Is a Major Symbiont of the Olive Fruit Fly (Bactrocera oleae). Appl. Environ. Microbiol. 75: 3281-3288 [Abstract] [Full Text]  
  • Huang, W. E., Ferguson, A., Singer, A. C., Lawson, K., Thompson, I. P., Kalin, R. M., Larkin, M. J., Bailey, M. J., Whiteley, A. S. (2009). Resolving Genetic Functions within Microbial Populations: In Situ Analyses Using rRNA and mRNA Stable Isotope Probing Coupled with Single-Cell Raman-Fluorescence In Situ Hybridization. Appl. Environ. Microbiol. 75: 234-241 [Abstract] [Full Text]  
  • Galkiewicz, J. P., Kellogg, C. A. (2008). Cross-Kingdom Amplification Using Bacteria-Specific Primers: Complications for Studies of Coral Microbial Ecology. Appl. Environ. Microbiol. 74: 7828-7831 [Abstract] [Full Text]  
  • Commons, R., Rogers, S., Gooding, T., Danchin, M., Carapetis, J., Robins-Browne, R., Curtis, N. (2008). Superantigen genes in group A streptococcal isolates and their relationship with emm types. J Med Microbiol 57: 1238-1246 [Abstract] [Full Text]  
  • Ponnusamy, L., Xu, N., Nojima, S., Wesson, D. M., Schal, C., Apperson, C. S. (2008). Identification of bacteria and bacteria-associated chemical cues that mediate oviposition site preferences by Aedes aegypti. Proc. Natl. Acad. Sci. USA 105: 9262-9267 [Abstract] [Full Text]  
  • Kugeler, K. J., Mead, P. S., McGowan, K. L., Burnham, J. M., Hogarty, M. D., Ruchelli, E., Pollard, K., Husband, B., Conley, C., Rivera, T., Kelesidis, T., Lee, W. M., Mabey, W., Winchell, J. M., Stang, H. L., Staples, J. E., Chalcraft, L. J., Petersen, J. M. (2008). Isolation and Characterization of a Novel Francisella sp. from Human Cerebrospinal Fluid and Blood. J. Clin. Microbiol. 46: 2428-2431 [Abstract] [Full Text]  
  • Richards, G. P., Watson, M. A., Crane, E. J. III, Burt, I. G., Bushek, D. (2008). Shewanella and Photobacterium spp. in Oysters and Seawater from the Delaware Bay. Appl. Environ. Microbiol. 74: 3323-3327 [Abstract] [Full Text]  
  • Qvit-Raz, N., Jurkevitch, E., Belkin, S. (2008). Drop-Size Soda Lakes: Transient Microbial Habitats on a Salt-Secreting Desert Tree. Genetics 178: 1615-1622 [Abstract] [Full Text]  
  • Schuler, L., Ni Chadhain, S. M., Jouanneau, Y., Meyer, C., Zylstra, G. J., Hols, P., Agathos, S. N. (2008). Characterization of a Novel Angular Dioxygenase from Fluorene-Degrading Sphingomonas sp. Strain LB126. Appl. Environ. Microbiol. 74: 1050-1057 [Abstract] [Full Text]  
  • Pruesse, E., Quast, C., Knittel, K., Fuchs, B. M., Ludwig, W., Peplies, J., Glockner, F. O. (2007). SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Res 35: 7188-7196 [Abstract] [Full Text]  
  • Savic, M., Bratic, I., Vasiljevic, B. (2007). Streptomyces durmitorensis sp. nov., a producer of an FK506-like immunosuppressant. Int. J. Syst. Evol. Microbiol. 57: 2119-2124 [Abstract] [Full Text]  
  • Eddy, F., Powell, A., Gregory, S., Nunan, L. M., Lightner, D. V., Dyson, P. J., Rowley, A. F., Shields, R. J. (2007). A novel bacterial disease of the European shore crab, Carcinus maenas molecular pathology and epidemiology. Microbiology 153: 2839-2849 [Abstract] [Full Text]  
  • Rodriguez-Echeverria, S., Crisostomo, J. A., Freitas, H. (2007). Genetic Diversity of Rhizobia Associated with Acacia longifolia in Two Stages of Invasion of Coastal Sand Dunes. Appl. Environ. Microbiol. 73: 5066-5070 [Abstract] [Full Text]  
  • Perotti, M. A., Allen, J. M., Reed, D. L., Braig, H. R. (2007). Host-symbiont interactions of the primary endosymbiont of human head and body lice. FASEB J. 21: 1058-1066 [Abstract] [Full Text]  
  • Thies, J. E. (2007). Soil Microbial Community Analysis using Terminal Restriction Fragment Length Polymorphisms. Soil Sci. 71: 579-591 [Abstract] [Full Text]  
  • Heimesaat, M. M., Bereswill, S., Fischer, A., Fuchs, D., Struck, D., Niebergall, J., Jahn, H.-K., Dunay, I. R., Moter, A., Gescher, D. M., Schumann, R. R., Gobel, U. B., Liesenfeld, O. (2006). Gram-Negative Bacteria Aggravate Murine Small Intestinal Th1-Type Immunopathology following Oral Infection with Toxoplasma gondii. J. Immunol. 177: 8785-8795 [Abstract] [Full Text]  
  • Singh, B. K., Nazaries, L., Munro, S., Anderson, I. C., Campbell, C. D. (2006). Use of Multiplex Terminal Restriction Fragment Length Polymorphism for Rapid and Simultaneous Analysis of Different Components of the Soil Microbial Community{triangledown}. Appl. Environ. Microbiol. 72: 7278-7285 [Abstract] [Full Text]  
  • Gophna, U., Sommerfeld, K., Gophna, S., Doolittle, W. F., Veldhuyzen van Zanten, S. J. O. (2006). Differences between Tissue-Associated Intestinal Microfloras of Patients with Crohn's Disease and Ulcerative Colitis. J. Clin. Microbiol. 44: 4136-4141 [Abstract] [Full Text]  
  • Perotti, M. A., Clarke, H. K., Turner, B. D., Braig, H. R. (2006). Rickettsia as obligate and mycetomic bacteria. FASEB J. 20: 2372-2374 [Abstract] [Full Text]  
  • Herrero, M., Quiros, C., Garcia, L. A., Diaz, M. (2006). Use of flow cytometry to follow the physiological States of microorganisms in cider fermentation processes.. Appl. Environ. Microbiol. 72: 6725-6733 [Abstract] [Full Text]  
  • Castillo, M., Martin-Orue, S. M., Roca, M., Manzanilla, E. G., Badiola, I., Perez, J. F., Gasa, J. (2006). The response of gastrointestinal microbiota to avilamycin, butyrate, and plant extracts in early-weaned pigs. J ANIM SCI 84: 2725-2734 [Abstract] [Full Text]  
  • Moss, J. A., Nocker, A., Lepo, J. E., Snyder, R. A. (2006). Stability and Change in Estuarine Biofilm Bacterial Community Diversity. Appl. Environ. Microbiol. 72: 5679-5688 [Abstract] [Full Text]  
  • Hayashi, S., Okura, M., Osawa, R. (2006). Soft-Agar-Coated Filter Method for Early Detection of Viable and Thermostable Direct Hemolysin (TDH)- or TDH-Related Hemolysin-Producing Vibrio parahaemolyticus in Seafood.. Appl. Environ. Microbiol. 72: 4576-4582 [Abstract] [Full Text]  
  • Stelzer, S., Egan, S., Larsen, M. R., Bartlett, D. H., Kjelleberg, S. (2006). Unravelling the role of the ToxR-like transcriptional regulator WmpR in the marine antifouling bacterium Pseudoalteromonas tunicata.. Microbiology 152: 1385-1394 [Abstract] [Full Text]  
  • Lucke, K., Miehlke, S., Jacobs, E., Schuppler, M. (2006). Prevalence of Bacteroides and Prevotella spp. in ulcerative colitis.. J Med Microbiol 55: 617-624 [Abstract] [Full Text]  
  • Gammon, K., Jones, G. W., Hope, S. J., de Oliveira, C. M. F., Regis, L., Silva Filha, M. H. N. L., Dancer, B. N., Berry, C. (2006). Conjugal Transfer of a Toxin-Coding Megaplasmid from Bacillus thuringiensis subsp. israelensis to Mosquitocidal Strains of Bacillus sphaericus.. Appl. Environ. Microbiol. 72: 1766-1770 [Abstract] [Full Text]  
  • de las Rivas, B., Marcobal, A., Munoz, R. (2006). Development of a multilocus sequence typing method for analysis of Lactobacillus plantarum strains. Microbiology 152: 85-93 [Abstract] [Full Text]  
  • Miteva, V. I., Brenchley, J. E. (2005). Detection and Isolation of Ultrasmall Microorganisms from a 120,000-Year-Old Greenland Glacier Ice Core. Appl. Environ. Microbiol. 71: 7806-7818 [Abstract] [Full Text]  
  • Noguchi, N., Noiri, Y., Narimatsu, M., Ebisu, S. (2005). Identification and Localization of Extraradicular Biofilm-Forming Bacteria Associated with Refractory Endodontic Pathogens. Appl. Environ. Microbiol. 71: 8738-8743 [Abstract] [Full Text]  
  • Kugeler, K. J., Gurfield, N., Creek, J. G., Mahoney, K. S., Versage, J. L., Petersen, J. M. (2005). Discrimination between Francisella tularensis and Francisella-Like Endosymbionts when Screening Ticks by PCR. Appl. Environ. Microbiol. 71: 7594-7597 [Abstract] [Full Text]  
  • Neufeld, J. D., Mohn, W. W. (2005). Fluorophore-Labeled Primers Improve the Sensitivity, Versatility, and Normalization of Denaturing Gradient Gel Electrophoresis. Appl. Environ. Microbiol. 71: 4893-4896 [Abstract] [Full Text]  
  • Sharma, A., Inagaki, S., Honma, K., Sfintescu, C., Baker, P.J., Evans, R.T. (2005). Tannerella forsythia-induced Alveolar Bone Loss in Mice Involves Leucine-rich-repeat BspA Protein. JDR 84: 462-467 [Abstract] [Full Text]  
  • Rosch, C., Bothe, H. (2005). Improved Assessment of Denitrifying, N2-Fixing, and Total-Community Bacteria by Terminal Restriction Fragment Length Polymorphism Analysis Using Multiple Restriction Enzymes. Appl. Environ. Microbiol. 71: 2026-2035 [Abstract] [Full Text]  
  • Chouari, R., Le Paslier, D., Dauga, C., Daegelen, P., Weissenbach, J., Sghir, A. (2005). Novel Major Bacterial Candidate Division within a Municipal Anaerobic Sludge Digester. Appl. Environ. Microbiol. 71: 2145-2153 [Abstract] [Full Text]  
  • Drgon, T., Saito, K., Gillevet, P. M., Sikaroodi, M., Whitaker, B., Krupatkina, D. N., Argemi, F., Vasta, G. R. (2005). Characterization of Ichthyocidal Activity of Pfiesteria piscicida: Dependence on the Dinospore Cell Density. Appl. Environ. Microbiol. 71: 519-529 [Abstract] [Full Text]  
  • Romero, P., Lopez, R., Garcia, E. (2004). Characterization of LytA-Like N-Acetylmuramoyl-L-Alanine Amidases from Two New Streptococcus mitis Bacteriophages Provides Insights into the Properties of the Major Pneumococcal Autolysin. J. Bacteriol. 186: 8229-8239 [Abstract] [Full Text]  
  • Boison, G., Mergel, A., Jolkver, H., Bothe, H. (2004). Bacterial Life and Dinitrogen Fixation at a Gypsum Rock. Appl. Environ. Microbiol. 70: 7070-7077 [Abstract] [Full Text]  
  • de las Rivas, B., Marcobal, A., Munoz, R. (2004). Allelic Diversity and Population Structure in Oenococcus oeni as Determined from Sequence Analysis of Housekeeping Genes. Appl. Environ. Microbiol. 70: 7210-7219 [Abstract] [Full Text]  
  • Webster, G., Parkes, R. J., Fry, J. C., Weightman, A. J. (2004). Widespread Occurrence of a Novel Division of Bacteria Identified by 16S rRNA Gene Sequences Originally Found in Deep Marine Sediments. Appl. Environ. Microbiol. 70: 5708-5713 [Abstract] [Full Text]  
  • Davidov, Y., Jurkevitch, E. (2004). Diversity and evolution of Bdellovibrio-and-like organisms (BALOs), reclassification of Bacteriovorax starrii as Peredibacter starrii gen. nov., comb. nov., and description of the Bacteriovorax-Peredibacter clade as Bacteriovoracaceae fam. nov.. Int. J. Syst. Evol. Microbiol. 54: 1439-1452 [Abstract] [Full Text]  
  • Bistrussu, S., Beeton, A., Castaldo, G., Han, J., Wong, I., Tuleu, C., Long, P. F., Brown, K., Cross, N., Cope, J., Goldman, A. P., Karimova, A., O'Callaghan, M., Robertson, A., Smith, L. (2004). Are Extracorporeal Membrane Oxygenation Circuits That Are Primed with Plasmalyte and Stored a Likely Source of Infection?. J. Clin. Microbiol. 42: 3906-3906 [Full Text]  
  • Leys, N. M. E. J., Ryngaert, A., Bastiaens, L., Verstraete, W., Top, E. M., Springael, D. (2004). Occurrence and Phylogenetic Diversity of Sphingomonas Strains in Soils Contaminated with Polycyclic Aromatic Hydrocarbons. Appl. Environ. Microbiol. 70: 1944-1955 [Abstract] [Full Text]  
  • Skovhus, T. L., Ramsing, N. B., Holmstrom, C., Kjelleberg, S., Dahllof, I. (2004). Real-Time Quantitative PCR for Assessment of Abundance of Pseudoalteromonas Species in Marine Samples. Appl. Environ. Microbiol. 70: 2373-2382 [Abstract] [Full Text]  
  • Schuppler, M., Lotzsch, K., Waidmann, M., Autenrieth, I. B. (2004). An Abundance of Escherichia coli Is Harbored by the Mucosa- Associated Bacterial Flora of Interleukin-2-Deficient Mice. Infect. Immun. 72: 1983-1990 [Abstract] [Full Text]  
  • Domann, E., Hong, G., Imirzalioglu, C., Turschner, S., Kuhle, J., Watzel, C., Hain, T., Hossain, H., Chakraborty, T. (2003). Culture-Independent Identification of Pathogenic Bacteria and Polymicrobial Infections in the Genitourinary Tract of Renal Transplant Recipients. J. Clin. Microbiol. 41: 5500-5510 [Abstract] [Full Text]  
  • Roy, A. B., Hewlins, M. J. E., Ellis, A. J., Harwood, J. L., White, G. F. (2003). Glycolytic Breakdown of Sulfoquinovose in Bacteria: a Missing Link in the Sulfur Cycle. Appl. Environ. Microbiol. 69: 6434-6441 [Abstract] [Full Text]  
  • Lopez, I., Ruiz-Larrea, F., Cocolin, L., Orr, E., Phister, T., Marshall, M., VanderGheynst, J., Mills, D. A. (2003). Design and Evaluation of PCR Primers for Analysis of Bacterial Populations in Wine by Denaturing Gradient Gel Electrophoresis. Appl. Environ. Microbiol. 69: 6801-6807 [Abstract] [Full Text]  
  • Saini, R., Halverson, L. J., Lorimor, J. C. (2003). Rainfall Timing and Frequency Influence on Leaching of Escherichia coli RS2G through Soil Following Manure Application. J. Environ. Qual. 32: 1865-1872 [Abstract] [Full Text]  
  • Paju, S., Bernstein, J. M., Haase, E. M., Scannapieco, F. A. (2003). Molecular analysis of bacterial flora associated with chronically inflamed maxillary sinuses. J Med Microbiol 52: 591-597 [Abstract] [Full Text]  
  • Hill, K.E., Davies, C.E., Wilson, M.J., Stephens, P., Harding, K.G., Thomas, D.W. (2003). Molecular analysis of the microflora in chronic venous leg ulceration. J Med Microbiol 52: 365-369 [Abstract] [Full Text]  
  • Dejonghe, W., Berteloot, E., Goris, J., Boon, N., Crul, K., Maertens, S., Hofte, M., De Vos, P., Verstraete, W., Top, E. M. (2003). Synergistic Degradation of Linuron by a Bacterial Consortium and Isolation of a Single Linuron-Degrading Variovorax Strain. Appl. Environ. Microbiol. 69: 1532-1541 [Abstract] [Full Text]  
  • Girvan, M. S., Bullimore, J., Pretty, J. N., Osborn, A. M., Ball, A. S. (2003). Soil Type Is the Primary Determinant of the Composition of the Total and Active Bacterial Communities in Arable Soils. Appl. Environ. Microbiol. 69: 1800-1809 [Abstract] [Full Text]  
  • Courtois, S., Cappellano, C. M., Ball, M., Francou, F.-X., Normand, P., Helynck, G., Martinez, A., Kolvek, S. J., Hopke, J., Osburne, M. S., August, P. R., Nalin, R., Guerineau, M., Jeannin, P., Simonet, P., Pernodet, J.-L. (2003). Recombinant Environmental Libraries Provide Access to Microbial Diversity for Drug Discovery from Natural Products. Appl. Environ. Microbiol. 69: 49-55 [Abstract] [Full Text]  
  • Hutter, G., Schlagenhauf, U., Valenza, G., Horn, M., Burgemeister, S., Claus, H., Vogel, U. (2003). Molecular analysis of bacteria in periodontitis: evaluation of clone libraries, novel phylotypes and putative pathogens. Microbiology 149: 67-75 [Abstract] [Full Text]  
  • HILL, K.E., DAVIES, C.E., WILSON, M.J., STEPHENS, P., LEWIS, M.A. O., HALL, V., BRAZIER, J., THOMAS., D.W. (2002). Heterogeneity within the gram-positive anaerobic cocci demonstrated by analysis of 16S-23S intergenic ribosomal RNA polymorphisms. J Med Microbiol 51: 949-957 [Abstract] [Full Text]  
  • Obregon, V., Garcia, P., Garcia, E., Fenoll, A., Lopez, R., Garcia, J. L. (2002). Molecular Peculiarities of the lytA Gene Isolated from Clinical Pneumococcal Strains That Are Bile Insoluble. J. Clin. Microbiol. 40: 2545-2554 [Abstract] [Full Text]  
  • Peng, X., Luo, W., Zhang, J., Wang, S., Lin, S. (2002). Rapid Detection of Shigella Species in Environmental Sewage by an Immunocapture PCR with Universal Primers. Appl. Environ. Microbiol. 68: 2580-2583 [Abstract] [Full Text]  
  • Bond, D. R., Holmes, D. E., Tender, L. M., Lovley, D. R. (2002). Electrode-Reducing Microorganisms That Harvest Energy from Marine Sediments. Science 295: 483-485 [Abstract] [Full Text]  
  • Zhu, X. Y., Zhong, T., Pandya, Y., Joerger, R. D. (2002). 16S rRNA-Based Analysis of Microbiota from the Cecum of Broiler Chickens. Appl. Environ. Microbiol. 68: 124-137 [Abstract] [Full Text]  
  • O'Sullivan, L. A., Weightman, A. J., Fry, J. C. (2002). New Degenerate Cytophaga-Flexibacter-Bacteroides-Specific 16S Ribosomal DNA-Targeted Oligonucleotide Probes Reveal High Bacterial Diversity in River Taff Epilithon. Appl. Environ. Microbiol. 68: 201-210 [Abstract] [Full Text]  
  • Nadkarni, M. A., Martin, F. E., Jacques, N. A., Hunter, N. (2002). Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology 148: 257-266 [Abstract] [Full Text]  
  • Rolph, H. J., Lennon, A., Riggio, M. P., Saunders, W. P., MacKenzie, D., Coldero, L., Bagg, J. (2001). Molecular Identification of Microorganisms from Endodontic Infections. J. Clin. Microbiol. 39: 3282-3289 [Abstract] [Full Text]  
  • Schmalenberger, A., Schwieger, F., Tebbe, C. C. (2001). Effect of Primers Hybridizing to Different Evolutionarily Conserved Regions of the Small-Subunit rRNA Gene in PCR-Based Microbial Community Analyses and Genetic Profiling. Appl. Environ. Microbiol. 67: 3557-3563 [Abstract] [Full Text]  
  • Marshall, S. J., White, G. F. (2001). Complete Denitration of Nitroglycerin by Bacteria Isolated from a Washwater Soakaway. Appl. Environ. Microbiol. 67: 2622-2626 [Abstract] [Full Text]  
  • Chiu, P. C., Lee, M. (2001). 2-Bromoethanesulfonate Affects Bacteria in a Trichloroethene-Dechlorinating Culture. Appl. Environ. Microbiol. 67: 2371-2374 [Abstract] [Full Text]  
  • Effendi, A. J., Greenaway, S. D., Dancer, B. N. (2000). Isolation and Characterization of 2,3-Dichloro-1-Propanol-Degrading Rhizobia. Appl. Environ. Microbiol. 66: 2882-2887 [Abstract] [Full Text]  
  • Jurkevitch, E., Minz, D., Ramati, B., Barel, G. (2000). Prey Range Characterization, Ribotyping, and Diversity of Soil and Rhizosphere Bdellovibrio spp. Isolated on Phytopathogenic Bacteria. Appl. Environ. Microbiol. 66: 2365-2371 [Abstract] [Full Text]  
  • Poelarends, G. J., Zandstra, M., Bosma, T., Kulakov, L. A., Larkin, M. J., Marchesi, J. R., Weightman, A. J., Janssen, D. B. (2000). Haloalkane-Utilizing Rhodococcus Strains Isolated from Geographically Distinct Locations Possess a Highly Conserved Gene Cluster Encoding Haloalkane Catabolism. J. Bacteriol. 182: 2725-2731 [Abstract] [Full Text]  
  • Dang, H., Lovell, C. R. (2000). Bacterial Primary Colonization and Early Succession on Surfaces in Marine Waters as Determined by Amplified rRNA Gene Restriction Analysis and Sequence Analysis of 16S rRNA Genes. Appl. Environ. Microbiol. 66: 467-475 [Abstract] [Full Text]  
  • Green, D. H., Wakeley, P. R., Page, A., Barnes, A., Baccigalupi, L., Ricca, E., Cutting, S. M. (1999). Characterization of Two Bacillus Probiotics. Appl. Environ. Microbiol. 65: 4288-4291 [Abstract] [Full Text]  
  • Glöckner, F. O., Fuchs, B. M., Amann, R. (1999). Bacterioplankton Compositions of Lakes and Oceans: a First Comparison Based on Fluorescence In Situ Hybridization. Appl. Environ. Microbiol. 65: 3721-3726 [Abstract] [Full Text]  
  • Hill, K. E., Marchesi, J. R., Weightman, A. J. (1999). Investigation of Two Evolutionarily Unrelated Halocarboxylic Acid Dehalogenase Gene Families. J. Bacteriol. 181: 2535-2547 [Abstract] [Full Text]  
  • Poelarends, G. J., van Hylckama Vlieg, J. E. T., Marchesi, J. R., Freitas Dos Santos, L. M., Janssen, D. B. (1999). Degradation of 1,2-Dibromoethane by Mycobacterium sp. Strain GP1. J. Bacteriol. 181: 2050-2058 [Abstract] [Full Text]  
  • el Fantroussi, S., Verschuere, L., Verstraete, W., Top, E. M. (1999). Effect of Phenylurea Herbicides on Soil Microbial Communities Estimated by Analysis of 16S rRNA Gene Fingerprints and Community-Level Physiological Profiles. Appl. Environ. Microbiol. 65: 982-988 [Abstract] [Full Text]  
  • van Hylckama Vlieg, J. E. T., Kingma, J., van den Wijngaard, A. J., Janssen, D. B. (1998). A Glutathione S-Transferase with Activity towards cis-1,2-Dichloroepoxyethane Is Involved in Isoprene Utilization by Rhodococcus sp. Strain AD45. Appl. Environ. Microbiol. 64: 2800-2805 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»