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Appl Environ Microbiol, March 1998, p. 1139-1142, Vol. 64, No. 3
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Hydrostatic Pressure Enhances Vital Staining with Carboxyfluorescein or Carboxydichlorofluorescein in Saccharomyces cerevisiae: Efficient Detection of Labeled Yeasts by Flow Cytometry

Fumiyoshi Abe^*

The DEEPSTAR Group, Japan Marine Science and Technology Center, Yokosuka 237, Japan

Received 11 September 1997/Accepted 18 December 1997

The extent of intracellular accumulation of the fluorescent dye carboxyfluorescein or carboxydichlorofluorescein (CDCF) in Saccharomyces cerevisiae was found to be increased 5- to 10-fold under a nonlethal hydrostatic pressure of 30 to 50 MPa. This observation was confirmed by analysis of individual labeled cells by flow cytometry. The pressure-induced enhancement of staining with CDCF required D-glucose and was markedly inhibited by 2-deoxy-D-glucose, suggesting that glucose metabolism has a role in the process.

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^* Mailing address: The DEEPSTAR Group, Japan Marine Science and Technology Center, 2-15 Natsushima-cho, Yokosuka 237, Japan. Phone: 81-468-675542. Fax: 81-468-666364. E-mail: abef{at}jamstec.go.jp.

This article has been cited by other articles:

• Abe, F., Horikoshi, K. (2000). Tryptophan Permease Gene TAT2 Confers High-Pressure Growth in Saccharomyces cerevisiae. Mol. Cell. Biol. 20: 8093-8102 [Abstract] [Full Text]