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Appl Environ Microbiol, March 1998, p. 1163-1165, Vol. 64, No. 3
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Expression of Psychrophilic Genes in Mesophilic Hosts: Assessment of the Folding State of a Recombinant alpha -Amylase

Georges Feller,* Olivier Le Bussy, and Charles Gerday

Laboratory of Biochemistry, Institute of Chemistry, University of Liège, B-4000 Liège-Sart Tilman, Belgium

Received 28 August 1997/Accepted 5 December 1997

alpha -Amylase from the antarctic psychrophile Alteromonas haloplanktis is synthesized at 0 ± 2°C by the wild strain. This heat-labile alpha -amylase folds correctly when overexpressed in Escherichia coli, providing the culture temperature is sufficiently low to avoid irreversible denaturation. In the described expression system, a compromise between enzyme stability and E. coli growth rate is reached at 18°C.

* Corresponding author. Mailing address: Laboratory of Biochemistry, Institute of Chemistry B6, University of Liège, B-4000 Liège-Sart Tilman, Belgium. Phone: 32-4-366 33 43. Fax: 32-4-366 33 64. E-mail: gfeller{at}ulg.ac.be.




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