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Appl Environ Microbiol, March 1998, p. 830-835, Vol. 64, No. 3
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

2-Chloro-1,4-Dimethoxybenzene as a Novel Catalytic Cofactor for Oxidation of Anisyl Alcohol by Lignin Peroxidase

Pauline J. M. Teunissen* and Jim A. Field

Department of Food Technology and Nutrition Sciences, Division of Industrial Microbiology, Wageningen Agricultural University, 6700 EV Wageningen, The Netherlands

Received 18 September 1997/Accepted 22 December 1997

2-Chloro-1,4-dimethoxybenzene (2Cl-14DMB) is a natural compound produced de novo by several white rot fungi. This chloroaromatic metabolite was identified as a cofactor superior to veratryl alcohol (VA) in the oxidation of anisyl alcohol (AA) by lignin peroxidase (LiP). Our results reveal that good LiP substrates, such as VA and tryptophan, are comparatively poor cofactors in the oxidation of AA. Furthermore, we show that a good cofactor does not necessarily serve a role in protecting LiP against H2O2 inactivation. 2Cl-14DMB was not a direct mediator of AA oxidation, since increasing AA concentrations did not inhibit the oxidation of 2Cl-14DMB at all. However, the high molar ratio of anisaldehyde formed to 2Cl-14DMB consumed, up to 13:1, indicates that a mechanism which recycles the cofactor is present.

* Corresponding author. Mailing address: Division of Industrial Microbiology, Department of Food Technology and Nutrition Sciences, Wageningen Agricultural University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands. Phone: 31317 484980 or -484749. Fax: 31317 484978. E-mail: Pauline.Teunissen{at}algemeen.im.wau.nl.






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