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Appl Environ Microbiol, April 1998, p. 1313-1318, Vol. 64, No. 4
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of mRNA by Reverse Transcription-PCR as an Indicator of Viability in Escherichia coli Cells

G. E. C. Sheridan, C. I. Masters, J. A. Shallcross,dagger and B. M. Mackey*

Institute of Food Research, Reading RG6 6BZ, United Kingdom

Received 22 October 1997/Accepted 26 January 1998

The relationship between the detection of mRNA and cellular viability in Escherichia coli was investigated in cells killed by heat or ethanol. Reverse transcription-PCR (RT-PCR) methods were developed for detecting mRNA from rpoH, groEL, and tufA genes. mRNA from all three genes was detected immediately after the cells had been killed by heat or ethanol but gradually disappeared with time when dead cells were held at room temperature. In heat-killed cells, some mRNA targets became undetectable after 2 to 16 h, whereas after ethanol treatment, mRNA was still detected after 16 h. In contrast, 16S rRNA was detected by RT-PCR in all samples containing dead cells and did not disappear during a subsequent incubation of 16 h at room temperature. Of the different types of nucleic acid, mRNA is the most promising candidate for an indicator of viability in bacteria, but its persistence in dead cells depends on the inactivating treatment and subsequent holding conditions.

* Corresponding author. Mailing address: Institute of Food Research, Earley Gate, Whiteknights Road, Reading, Berks RG6 6BZ, United Kingdom. Phone: 44 1189 357230. Fax: 44 1189 357222.

dagger Present address: CAMR, Porton Down, Salisbury, SP4 0JG, United Kingdom.




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