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Appl Environ Microbiol, April 1998, p. 1412-1419, Vol. 64, No. 4
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Cloning and Transcriptional Regulation of the Aspergillus nidulans xlnD Gene Encoding a beta -Xylosidase

José A. Pérez-González,1,dagger Noël N. M. E. van Peij,2 Alja Bezoen,2 Andrew P. Maccabe,1 Daniel Ramón,1,* and Leo H. de Graaff2

Departamento de Biotecnología de los Alimentos, Instituto de Agroquímica y Tecnología de los Alimentos, Consejo Superior de Investigaciones Científicas, 46100 Burjassot, Valencia, Spain,1 and Molecular Genetics of Industrial Microorganisms, Wageningen Agricultural University, 6703 HA Wageningen, The Netherlands2

Received 16 June 1997/Accepted 25 January 1998

The xlnD gene encoding the 85-kDa beta -xylosidase was cloned from Aspergillus nidulans. The deduced primary structure of the protein exhibits considerable similarity to the primary structures of the Aspergillus niger and Trichoderma reesei beta -xylosidases and some similarity to the primary structures of the class 3 beta -glucosidases. xlnD is regulated at the transcriptional level; it is induced by xylan and D-xylose and is repressed by D-glucose. Glucose repression is mediated by the product of the creA gene. Although several binding sites for the pH regulatory protein PacC were found in the upstream regulatory region, it was not clear from a Northern analysis whether PacC is involved in transcriptional regulation of xlnD.

* Corresponding author. Mailing address: Departamento de Biotecnología de los Alimentos, Instituto de Agroquímica y Tecnología de los Alimentos, Apartado Postal 73, 46100 Burjassot, Valencia, Spain. Phone: 34-6-3900022. Fax: 34-6-3636301. E-mail: dramon{at}iata.csic.es.

dagger Deceased 9 August 1997.




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