This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cavin, J.-F.
Right arrow Articles by Diviès, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cavin, J.-F.
Right arrow Articles by Diviès, C.
Agricola
Right arrow Articles by Cavin, J.-F.
Right arrow Articles by Diviès, C.

 Previous Article  |  Next Article 

Appl Environ Microbiol, April 1998, p. 1466-1471, Vol. 64, No. 4
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Gene Cloning, Transcriptional Analysis, Purification, and Characterization of Phenolic Acid Decarboxylase from Bacillus subtilis

Jean-François Cavin,* Véronique Dartois,dagger and Charles Diviès

Laboratoire de Microbiologie U.A. INRA, ENSBANA, Université de Bourgogne, 21000 Dijon, France

Received 27 October 1997/Accepted 26 January 1998

Bacillus subtilis displays a substrate-inducible decarboxylating activity with the following three phenolic acids: ferulic, p-coumaric, and caffeic acids. Based on DNA sequence homologies between the Bacillus pumilus ferulate decarboxylase gene (fdc) (A. Zago, G. Degrassi, and C. V. Bruschi, Appl. Environ. Microbiol. 61:4484-4486, 1995) and the Lactobacillus plantarum p-coumarate decarboxylase gene (pdc) (J.-F. Cavin, L. Barthelmebs, and C. Diviès, Appl. Environ. Microbiol. 63:1939-1944, 1997), a DNA probe of about 300 nucleotides for the L. plantarum pdc gene was used to screen a B. subtilis genomic library in order to clone the corresponding gene in this bacterium. One clone was detected with this heterologous probe, and this clone exhibited phenolic acid decarboxylase (PAD) activity. The corresponding 5-kb insertion was partially sequenced and was found to contain a 528-bp open reading frame coding for a 161-amino-acid protein exhibiting 71 and 84% identity with the pdc- and fdc-encoded enzymes, respectively. The PAD gene (pad) is transcriptionally regulated by p-coumaric, ferulic, or caffeic acid; these three acids are the three substrates of PAD. The pad gene was overexpressed constitutively in Escherichia coli, and the stable purified enzyme was characterized. The difference in substrate specificity between this PAD and other PADs seems to be related to a few differences in the amino acid sequence. Therefore, this novel enzyme should facilitate identification of regions involved in catalysis and substrate specificity.

* Corresponding author. Mailing address: Laboratoire de Microbiologie U.A. INRA, ENSBANA, 1 esplanade Erasme, F-21000 Dijon, France. Phone: (33) 03.80.39.66.72. Fax: (33) 03.80.39.66.40. E-mail: cavinjf{at}u-bourgogne.fr.

dagger Present address: Unité de Biochimie Microbienne, Institut Pasteur, 75724 Paris Cedex 15, France.




This article has been cited by other articles:

  • Tran, N. P., Gury, J., Dartois, V., Nguyen, T. K. C., Seraut, H., Barthelmebs, L., Gervais, P., Cavin, J.-F. (2008). Phenolic Acid-Mediated Regulation of the padC Gene, Encoding the Phenolic Acid Decarboxylase of Bacillus subtilis. J. Bacteriol. 190: 3213-3224 [Abstract] [Full Text]  
  • Beek, A. T., Keijser, B. J. F., Boorsma, A., Zakrzewska, A., Orij, R., Smits, G. J., Brul, S. (2008). Transcriptome Analysis of Sorbic Acid-Stressed Bacillus subtilis Reveals a Nutrient Limitation Response and Indicates Plasma Membrane Remodeling. J. Bacteriol. 190: 1751-1761 [Abstract] [Full Text]  
  • Stratford, M., Plumridge, A., Archer, D. B. (2007). Decarboxylation of Sorbic Acid by Spoilage Yeasts Is Associated with the PAD1 Gene. Appl. Environ. Microbiol. 73: 6534-6542 [Abstract] [Full Text]  
  • Schuhle, K., Fuchs, G. (2004). Phenylphosphate Carboxylase: a New C-C Lyase Involved in Anaerobic Phenol Metabolism in Thauera aromatica. J. Bacteriol. 186: 4556-4567 [Abstract] [Full Text]  
  • Gury, J., Barthelmebs, L., Tran, N. P., Divies, C., Cavin, J.-F. (2004). Cloning, Deletion, and Characterization of PadR, the Transcriptional Repressor of the Phenolic Acid Decarboxylase-Encoding padA Gene of Lactobacillus plantarum. Appl. Environ. Microbiol. 70: 2146-2153 [Abstract] [Full Text]  
  • Peng, X., Misawa, N., Harayama, S. (2003). Isolation and Characterization of Thermophilic Bacilli Degrading Cinnamic, 4-Coumaric, and Ferulic Acids. Appl. Environ. Microbiol. 69: 1417-1427 [Abstract] [Full Text]  
  • Barthelmebs, L., Diviès, C., Cavin, J.-F. (2001). Expression in Escherichia coli of Native and Chimeric Phenolic Acid Decarboxylases with Modified Enzymatic Activities and Method for Screening Recombinant E. coli Strains Expressing These Enzymes. Appl. Environ. Microbiol. 67: 1063-1069 [Abstract] [Full Text]  
  • van Beek, S., Priest, F. G. (2000). Decarboxylation of Substituted Cinnamic Acids by Lactic Acid Bacteria Isolated during Malt Whisky Fermentation. Appl. Environ. Microbiol. 66: 5322-5328 [Abstract] [Full Text]  
  • Barthelmebs, L., Lecomte, B., Divies, C., Cavin, J.-F. (2000). Inducible Metabolism of Phenolic Acids in Pediococcus pentosaceus Is Encoded by an Autoregulated Operon Which Involves a New Class of Negative Transcriptional Regulator. J. Bacteriol. 182: 6724-6731 [Abstract] [Full Text]  
  • Breinig, S., Schiltz, E., Fuchs, G. (2000). Genes Involved in Anaerobic Metabolism of Phenol in the Bacterium Thauera aromatica. J. Bacteriol. 182: 5849-5863 [Abstract] [Full Text]  
  • Herbelin, C. J., Chirillo, S. C., Melnick, K. A., Whittam, T. S. (2000). Gene Conservation and Loss in the mutS-rpoS Genomic Region of Pathogenic Escherichia coli. J. Bacteriol. 182: 5381-5390 [Abstract] [Full Text]  
  • Barthelmebs, L., Divies, C., Cavin, J.-F. (2000). Knockout of the p-Coumarate Decarboxylase Gene from Lactobacillus plantarum Reveals the Existence of Two Other Inducible Enzymatic Activities Involved in Phenolic Acid Metabolism. Appl. Environ. Microbiol. 66: 3368-3375 [Abstract] [Full Text]  
  • Huang, J., He, 2., Wiegel, J. (1999). Cloning, Characterization, and Expression of a Novel Gene Encoding a Reversible 4-Hydroxybenzoate Decarboxylase from Clostridium hydroxybenzoicum. J. Bacteriol. 181: 5119-5122 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»