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Appl Environ Microbiol, April 1998, p. 1532-1535, Vol. 64, No. 4
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Enterohemorrhagic Escherichia coli O157:H7 Present in Radish Sprouts

Yoshinori Itoh,1 Yoshiko Sugita-Konishi,1 Fumiko Kasuga,1 Masaaki Iwaki,1 Yukiko Hara-Kudo,1 Noriko Saito,2 Yoko Noguchi,3 Hirotaka Konuma,4 and Susumu Kumagai1,*

Department of Biomedical Food Research,1 Laboratory of Electron Microscopy,2 and Department of Veterinary Science,3 National Institute of Infectious Diseases, Shinjuku-ku, and Division of Microbiology, National Institute of Health Sciences, Setagaya-ku,4 Tokyo 162, Japan

Received 8 December 1997/Accepted 9 January 1998

Using cultivation, immunofluorescence microscopy, and scanning electron microscopy, we demonstrated the presence of viable enterohemorrhagic Escherichia coli O157:H7 not only on the outer surfaces but also in the inner tissues and stomata of cotyledons of radish sprouts grown from seeds experimentally contaminated with the bacterium. HgCl2 treatment of the outer surface of the hypocotyl did not kill the contaminating bacteria, which emphasized the importance of either using seeds free from E. coli O157:H7 in the production of radish sprouts or heating the sprouts before they are eaten.

* Corresponding author. Mailing address: Department of Biomedical Food Research, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162, Japan. Phone: 81-3-5285-1111, ext. 2300. Fax: 81-3-5285-1176. E-mail: skumagai{at}nih.go.jp.




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