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Appl Environ Microbiol, April 1998, p. 1550-1554, Vol. 64, No. 4
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Transformation of Acinetobacter sp. Strain BD413 by Transgenic Sugar Beet DNA

Frank Gebhard and Kornelia Smalla*

Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Biochemie und Pflanzenvirologie, D-38104 Braunschweig, Germany

Received 22 September 1997/Accepted 20 January 1998

The ability of Acinetobacter sp. strain BD413(pFG4Delta nptII) to take up and integrate transgenic plant DNA based on homologous recombination was studied under optimized laboratory conditions. Restoration of nptII, resulting in kanamycin-resistant transformants, was observed with plasmid DNA, plant DNA, and homogenates carrying the gene nptII. Molecular analysis showed that some transformants not only restored the 317-bp deletion but also obtained additional DNA.

* Corresponding author. Mailing address: Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Biochemie und Pflanzenvirologie, Messeweg 11-12, D-38104 Braunschweig, Germany. Phone: 49 531 2993814. Fax: 49 531 2993013. E-mail: K.Smalla{at}bba.de.




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