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Appl Environ Microbiol, April 1998, p. 1569-1572, Vol. 64, No. 4
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Laser Microsurgery Permits Fungal Plasma Membrane Single-Ion-Channel Resolution at the Hyphal Tip

Anne-Aliénor Véry* and Julia M. Davies

Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom

Received 20 June 1997/Accepted 26 January 1998

A method for formation of high-electrical-resistance seals on the Neurospora crassa plasma membrane, allowing resolution of single-ion-channel activity by patch clamp electrophysiology, is reported. Laser microsurgery permits access to the hyphal apex without enzymatic cell wall digestion and loss of morphological polarity. Cell wall reformation is delayed by brefeldin. This method can allow full characterization of apical plasma membrane channels, which are implicated in tip growth.

* Corresponding author. Mailing address: Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, United Kingdom. Phone: (44) 1223 333 939. Fax: (44) 1223 333 953. E-mail: aav22{at}cam.ac.uk.




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