This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mäntynen, V.
Right arrow Articles by Lindström, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mäntynen, V.
Right arrow Articles by Lindström, K.
Agricola
Right arrow Articles by Mäntynen, V.
Right arrow Articles by Lindström, K.

 Previous Article  |  Next Article 

Appl Environ Microbiol, May 1998, p. 1634-1639, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Rapid PCR-Based DNA Test for Enterotoxic Bacillus cereus

Vesa Mäntynen* and Kristina Lindström

Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, Biocenter, FIN-00014 University of Helsinki, Finland

Received 19 November 1997/Accepted 23 February 1998

The occurrence of DNA sequences encoding the hemolysin HblA complex and Bacillus cereus enterotoxin BceT, which have recently been confirmed as enterotoxins, was studied in Bacillus spp. To amplify these DNA sequences, PCR primer systems for the B component of hblA and for bceT DNA sequences were developed. The results from the amplification of hblA sequences correlated well with results obtained with the B. cereus enterotoxin (diarrheal type) test kit (RPLA kit), but not with the results of the Bacillus diarrheal enterotoxin visual immunoassay (BDE kit). Except for two thermophilic strains, all strains that were positive in PCR amplification assays with the hblA primers were also positive when tested with the RPLA kit. The hblA DNA sequence was found in 33 strains, and these strains were closely related according to 16S rDNA-RFLP analysis, except B. pasteurii. In PCR amplifications with the bceT primers only the model strain gave a positive signal. It is concluded that screening of the hemolysin HblA complex by the PCR method allows faster detection of enterotoxin production than does testing with the RPLA enterotoxin kit.

* Corresponding author. Mailing address: Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, Biocenter, P.O. Box 56, FIN-00014 University of Helsinki, Finland. Phone: 358-9-70859277. Fax: 358-9-70859322. E-mail: Vesa.Mantynen{at}Helsinki.Fi.

Appl Environ Microbiol, May 1998, p. 1634-1639, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Zahner, V., Cabral, D. A., Regua-Mangia, A. H., Rabinovitch, L., Moreau, G., McIntosh, D. (2005). Distribution of Genes Encoding Putative Virulence Factors and Fragment Length Polymorphisms in the vrrA Gene among Brazilian Isolates of Bacillus cereus and Bacillus thuringiensis. Appl. Environ. Microbiol. 71: 8107-8114 [Abstract] [Full Text]  
  • Phelps, R. J., McKillip, J. L. (2002). Enterotoxin Production in Natural Isolates of Bacillaceae outside the Bacillus cereus Group. Appl. Environ. Microbiol. 68: 3147-3151 [Abstract] [Full Text]  
  • Rowan, N. J., Deans, K., Anderson, J. G., Gemmell, C. G., Hunter, I. S., Chaithong, T. (2001). Putative Virulence Factor Expression by Clinical and Food Isolates of Bacillus spp. after Growth in Reconstituted Infant Milk Formulae. Appl. Environ. Microbiol. 67: 3873-3881 [Abstract] [Full Text]  
  • Hansen, B. M., Hendriksen, N. B. (2001). Detection of Enterotoxic Bacillus cereus and Bacillus thuringiensis Strains by PCR Analysis. Appl. Environ. Microbiol. 67: 185-189 [Abstract] [Full Text]  
  • Charni, N., Perissol, C., Le Petit, J., Rugani, N. (2000). Production and Characterization of Monoclonal Antibodies against Vegetative Cells of Bacillus cereus. Appl. Environ. Microbiol. 66: 2278-2281 [Abstract] [Full Text]  
  • Prüß, B. M., Dietrich, R., Nibler, B., Märtlbauer, E., Scherer, S. (1999). The Hemolytic Enterotoxin HBL Is Broadly Distributed among Species of the Bacillus cereus Group. Appl. Environ. Microbiol. 65: 5436-5442 [Abstract] [Full Text]  
  • Yamada, S., Ohashi, E., Agata, N., Venkateswaran, K. (1999). Cloning and Nucleotide Sequence Analysis of gyrB of Bacillus cereus, B. thuringiensis, B. mycoides, and B. anthracis and Their Application to the Detection of B. cereus in Rice. Appl. Environ. Microbiol. 65: 1483-1490 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»