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Appl Environ Microbiol, May 1998, p. 1669-1672, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A Flow Cytometric Method for Rapid Selection of Novel Industrial Yeast Hybrids

P. J. L. Bell,1 D. Deere,2 J. Shen,1 B. Chapman,2 P. H. Bissinger,1,dagger P. V. Attfield,1 and D. A. Veal2,*

Burns Philp Technology and Research Centre, North Ryde, NSW 2113,1 and Flow Cytometry Group, School of Biological Sciences, Macquarie University, Sydney, NSW 2109,2 Australia

Received 1 August 1997/Accepted 17 February 1998

We rapidly produced and isolated novel yeast hybrids by using two-color flow cytometric cell sorting. We labeled one parent strain with a fluorescent green stain and the other parent with a fluorescent orange stain, and hybrids were selected based on their dual orange and green fluorescence. When this technique was applied to the production of hybrids by traditional mating procedures, more than 96% of the isolates were hybrids. When it was applied to rare mating, three hybrids were identified among 50 isolates enriched from a population containing 2 × 106 cells. This technology is not dependent on genetic markers and has applications in the development of improved industrial yeast strains.

* Corresponding author. Mailing address: School of Biological Sciences, Macquarie University, Sydney, NSW 2109, Australia. Phone: 612 9850 8157. Fax: 612 9850 8253. E-mail: DVEAL{at}RNA.BIO.MQ.EDU.AU.

dagger Present address: Institute of Molecular Agrobiology, Singapore 118240.

Appl Environ Microbiol, May 1998, p. 1669-1672, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Higgins, V. J., Bell, P. J. L., Dawes, I. W., Attfield, P. V. (2001). Generation of a Novel Saccharomyces cerevisiae Strain That Exhibits Strong Maltose Utilization and Hyperosmotic Resistance Using Nonrecombinant Techniques. Appl. Environ. Microbiol. 67: 4346-4348 [Abstract] [Full Text]  


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