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Appl Environ Microbiol, May 1998, p. 1725-1730, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of Blue Nucleic Acid Dyes for Flow Cytometric Enumeration of Bacteria in Aquatic Systems

Philippe Lebaron,* Nathalie Parthuisot, and Philippe Catala

Observatoire Océanologique, Centre National de la recherche Scientifique, Institut National des Sciences de l'Univers et Université Pierre et Marie Curie, F-66651 Banyuls-sur-Mer Cedex, France

Received 29 October 1997/Accepted 9 March 1998

Seven blue nucleic acid dyes from Molecular Probes Inc. (SYTO-9, SYTO-11, SYTO-13, SYTO-16, SYTO-BC, SYBR-I and SYBR-II) were compared with the DAPI (4',6-diamidino-2-phenylindole) method for flow cytometric enumeration of live and fixed bacteria in aquatic systems. It was shown that SYBR-II and SYTO-9 are the most appropriate dyes for bacterial enumeration in nonsaline waters and can be applied to both live and dead bacteria. The fluorescence signal/noise ratio was improved when SYTO-9 was used to stain living bacteria in nonsaline waters. Inversely, SYBR-II is more appropriate than SYTO dyes for bacterial enumeration of unfixed and fixed seawater samples.

* Corresponding author. Mailing address: Laboratoire ARAGO, BP44, F-66651 Banyuls-sur-Mer Cedex, France. Phone: (33) 4 68 88 73 53. Fax: (33) 4 68 88 73 95. E-mail: lebaron{at}arago.obs-banyuls.fr.

Appl Environ Microbiol, May 1998, p. 1725-1730, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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