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Appl Environ Microbiol, May 1998, p. 1816-1821, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evidence of Genomic Instability in Campylobacter jejuni Isolated from Poultry

Trudy M. Wassenaar,1,* Barbara Geilhausen,2 and Diane G. Newell3

Institute of Medical Microbiology and Hygiene, Johannes Gutenberg University, 55101 Mainz,1 and Institute of Medical Microbiology and Hygiene, University of Cologne, 50935 Cologne,2 Germany and Central Veterinary Laboratory (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, United Kingdom3

Received 26 November 1997/Accepted 2 March 1998

Poultry isolates of Campylobacter jejuni derived from a survey of meat processing batches were genotyped by pulsed-field gel electrophoresis (PFGE) of chromosomal DNA to establish the clonal relationships between single-colony isolates. In the majority of batches studied, one or two genotype patterns predominated. However, in one batch (batch A), 21 single-colony isolates gave 14 different PFGE genotypes. The banding patterns obtained with SmaI were sufficiently different to distinguish between genotypes, although the patterns also produced many common bands. The question of whether these isolates represented different clones or had a common clonal ancestry was addressed by additional genotypic and phenotypic methods. Restriction length polymorphism of PCR products obtained from the flagellin genes showed an identical flagellin genotype for all of these isolates. In contrast, unrelated control isolates resulted in different flagellin genotypes. Moreover, all 14 different PFGE genotypes of batch A had identical Penner serotypes and identical or similar biotypes and phage types. It was concluded that the isolates were of clonal origin and that the diversity in the PFGE banding patterns had most likely originated from genomic rearrangements. However, the PFGE genotypes were shown to be stable upon subculturing in vitro and after in vivo passage in chickens, and natural transformation between isogenic mutants carrying antibiotic markers did not occur in vivo in a chick colonization model. The possible mechanisms for the hypothesized genomic recombinations and the conditions that allow, induce, or select for such events are discussed.

* Corresponding author. Mailing address: Institute of Medical Microbiology and Hygiene, Johannes Gutenberg University, Hochhaus am Augustusplatz, 55101 Mainz, Germany. Phone: 6131 172865. Fax: 6131 392359. E-mail: Wassen{at}mzdmza.zdv.uni-mainz.de.

Appl Environ Microbiol, May 1998, p. 1816-1821, Vol. 64, No. 5
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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