This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Andersen, J. B.
Right arrow Articles by Molin, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Andersen, J. B.
Right arrow Articles by Molin, S.
Agricola
Right arrow Articles by Andersen, J. B.
Right arrow Articles by Molin, S.

 Previous Article  |  Next Article 

Appl Environ Microbiol, June 1998, p. 2240-2246, Vol. 64, No. 6
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria

Jens Bo Andersen,1 Claus Sternberg,1 Lars Kongsbak Poulsen,2 Sara Petersen Bjørn,3 Michael Givskov,1 and Søren Molin1,*

Department of Microbiology, The Technical University of Denmark, DK-2800 Lyngby,1 Microbial Discovery, Novo Nordisk A/S, DK-2880 Bagsværd,2 and Bio Image, Novo Nordisk A/S, Mørkhøj Bygade 28, DK-2860 Søborg,3 Denmark

Received 5 December 1997/Accepted 29 March 1998

Use of the green fluorescent protein (Gfp) from the jellyfish Aequorea victoria is a powerful method for nondestructive in situ monitoring, since expression of green fluorescence does not require any substrate addition. To expand the use of Gfp as a reporter protein, new variants have been constructed by the addition of short peptide sequences to the C-terminal end of intact Gfp. This rendered the Gfp susceptible to the action of indigenous housekeeping proteases, resulting in protein variants with half-lives ranging from 40 min to a few hours when synthesized in Escherichia coli and Pseudomonas putida. The new Gfp variants should be useful for in situ studies of temporal gene expression.

* Corresponding author. Mailing address: Department of Microbiology, Building 301, The Technical University of Denmark, DK-2800 Lyngby, Denmark. Phone: 45 45 25 25 13. Fax: 45 45 88 73 28. E-mail: sm{at}im.dtu.dk.

Appl Environ Microbiol, June 1998, p. 2240-2246, Vol. 64, No. 6
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Mao, X.-M., Zhou, Z., Hou, X.-P., Guan, W.-J., Li, Y.-Q. (2009). Reciprocal Regulation between SigK and Differentiation Programs in Streptomyces coelicolor. J. Bacteriol. 191: 6473-6481 [Abstract] [Full Text]  
  • Ind, A. C., Porter, S. L., Brown, M. T., Byles, E. D., de Beyer, J. A., Godfrey, S. A., Armitage, J. P. (2009). Inducible-Expression Plasmid for Rhodobacter sphaeroides and Paracoccus denitrificans. Appl. Environ. Microbiol. 75: 6613-6615 [Abstract] [Full Text]  
  • Yankson, K. K., Steck, T. R. (2009). Strategy for Extracting DNA from Clay Soil and Detecting a Specific Target Sequence via Selective Enrichment and Real-Time (Quantitative) PCR Amplification. Appl. Environ. Microbiol. 75: 6017-6021 [Abstract] [Full Text]  
  • Uehlinger, S., Schwager, S., Bernier, S. P., Riedel, K., Nguyen, D. T., Sokol, P. A., Eberl, L. (2009). Identification of Specific and Universal Virulence Factors in Burkholderia cenocepacia Strains by Using Multiple Infection Hosts. Infect. Immun. 77: 4102-4110 [Abstract] [Full Text]  
  • Strovas, T. J., Lidstrom, M. E. (2009). Population heterogeneity in Methylobacterium extorquens AM1. Microbiology 155: 2040-2048 [Abstract] [Full Text]  
  • Friedland, A. E., Lu, T. K., Wang, X., Shi, D., Church, G., Collins, J. J. (2009). Synthetic Gene Networks That Count. Science 324: 1199-1202 [Abstract] [Full Text]  
  • Malott, R. J., O'Grady, E. P., Toller, J., Inhulsen, S., Eberl, L., Sokol, P. A. (2009). A Burkholderia cenocepacia Orphan LuxR Homolog Is Involved in Quorum-Sensing Regulation. J. Bacteriol. 191: 2447-2460 [Abstract] [Full Text]  
  • Sastalla, I., Chim, K., Cheung, G. Y. C., Pomerantsev, A. P., Leppla, S. H. (2009). Codon-Optimized Fluorescent Proteins Designed for Expression in Low-GC Gram-Positive Bacteria. Appl. Environ. Microbiol. 75: 2099-2110 [Abstract] [Full Text]  
  • Perrin, C., Briandet, R., Jubelin, G., Lejeune, P., Mandrand-Berthelot, M.-A., Rodrigue, A., Dorel, C. (2009). Nickel Promotes Biofilm Formation by Escherichia coli K-12 Strains That Produce Curli. Appl. Environ. Microbiol. 75: 1723-1733 [Abstract] [Full Text]  
  • Kain, J., He, G. G., Losick, R. (2008). Polar Localization and Compartmentalization of ClpP Proteases during Growth and Sporulation in Bacillus subtilis. J. Bacteriol. 190: 6749-6757 [Abstract] [Full Text]  
  • Chang, Y., Wesenberg, G. E., Bingman, C. A., Fox, B. G. (2008). In Vivo Inactivation of the Mycobacterial Integral Membrane Stearoyl Coenzyme A Desaturase DesA3 by a C-Terminus-Specific Degradation Process. J. Bacteriol. 190: 6686-6696 [Abstract] [Full Text]  
  • Barysheva, O. V., Fujii, J., Takaesu, G., Yoshida, S.-i. (2008). Application of unstable Gfp variants to the kinetic study of Legionella pneumophila icm gene expression during infection. Microbiology 154: 1015-1025 [Abstract] [Full Text]  
  • Weber, B., Croxatto, A., Chen, C., Milton, D. L. (2008). RpoS induces expression of the Vibrio anguillarum quorum-sensing regulator VanT. Microbiology 154: 767-780 [Abstract] [Full Text]  
  • de Werra, P., Baehler, E., Huser, A., Keel, C., Maurhofer, M. (2008). Detection of Plant-Modulated Alterations in Antifungal Gene Expression in Pseudomonas fluorescens CHA0 on Roots by Flow Cytometry. Appl. Environ. Microbiol. 74: 1339-1349 [Abstract] [Full Text]  
  • Haseltine, E. L., Arnold, F. H. (2008). Implications of Rewiring Bacterial Quorum Sensing. Appl. Environ. Microbiol. 74: 437-445 [Abstract] [Full Text]  
  • Choy, J. S., Aung, L. L., Karzai, A. W. (2007). Lon Protease Degrades Transfer-Messenger RNA-Tagged Proteins. J. Bacteriol. 189: 6564-6571 [Abstract] [Full Text]  
  • Wilks, J. C., Slonczewski, J. L. (2007). pH of the Cytoplasm and Periplasm of Escherichia coli: Rapid Measurement by Green Fluorescent Protein Fluorimetry. J. Bacteriol. 189: 5601-5607 [Abstract] [Full Text]  
  • McKean, S. C., Davies, J. K., Moore, R. J. (2007). Expression of phospholipase D, the major virulence factor of Corynebacterium pseudotuberculosis, is regulated by multiple environmental factors and plays a role in macrophage death. Microbiology 153: 2203-2211 [Abstract] [Full Text]  
  • Yang, L., Barken, K. B., Skindersoe, M. E., Christensen, A. B., Givskov, M., Tolker-Nielsen, T. (2007). Effects of iron on DNA release and biofilm development by Pseudomonas aeruginosa. Microbiology 153: 1318-1328 [Abstract] [Full Text]  
  • Halle, S., Bumann, D., Herbrand, H., Willer, Y., Dahne, S., Forster, R., Pabst, O. (2007). Solitary Intestinal Lymphoid Tissue Provides a Productive Port of Entry for Salmonella enterica Serovar Typhimurium. Infect. Immun. 75: 1577-1585 [Abstract] [Full Text]  
  • Tu, K. C., Bassler, B. L. (2007). Multiple small RNAs act additively to integrate sensory information and control quorum sensing in Vibrio harveyi. Genes Dev. 21: 221-233 [Abstract] [Full Text]  
  • Haagensen, J. A. J., Klausen, M., Ernst, R. K., Miller, S. I., Folkesson, A., Tolker-Nielsen, T., Molin, S. (2007). Differentiation and Distribution of Colistin- and Sodium Dodecyl Sulfate-Tolerant Cells in Pseudomonas aeruginosa Biofilms. J. Bacteriol. 189: 28-37 [Abstract] [Full Text]  
  • Jackson, S. A., Koduvayur, S., Woodson, S. A. (2006). Self-splicing of a group I intron reveals partitioning of native and misfolded RNA populations in yeast. RNA 12: 2149-2159 [Abstract] [Full Text]  
  • Teal, T. K., Lies, D. P., Wold, B. J., Newman, D. K. (2006). Spatiometabolic Stratification of Shewanella oneidensis Biofilms. Appl. Environ. Microbiol. 72: 7324-7330 [Abstract] [Full Text]  
  • Moons, P., Van Houdt, R., Aertsen, A., Vanoirbeek, K., Engelborghs, Y., Michiels, C. W. (2006). Role of Quorum Sensing and Antimicrobial Component Production by Serratia plymuthica in Formation of Biofilms, Including Mixed Biofilms with Escherichia coli. Appl. Environ. Microbiol. 72: 7294-7300 [Abstract] [Full Text]  
  • Waters, C. M., Bassler, B. L. (2006). The Vibrio harveyi quorum-sensing system uses shared regulatory components to discriminate between multiple autoinducers. Genes Dev. 20: 2754-2767 [Abstract] [Full Text]  
  • Maruyama, F., Tani, K., Kenzaka, T., Yamaguchi, N., Nasu, M. (2006). Quantitative Determination of Free-DNA Uptake in River Bacteria at the Single-Cell Level by In Situ Rolling-Circle Amplification. Appl. Environ. Microbiol. 72: 6248-6256 [Abstract] [Full Text]  
  • Junker, L. M., Peters, J. E., Hay, A. G. (2006). Global analysis of candidate genes important for fitness in a competitive biofilm using DNA-array-based transposon mapping.. Microbiology 152: 2233-2245 [Abstract] [Full Text]  
  • Wopperer, J., Cardona, S. T., Huber, B., Jacobi, C. A., Valvano, M. A., Eberl, L. (2006). A Quorum-Quenching Approach To Investigate the Conservation of Quorum-Sensing-Regulated Functions within the Burkholderia cepacia Complex. Appl. Environ. Microbiol. 72: 1579-1587 [Abstract] [Full Text]  
  • Piao, Z., Sze, C. C., Barysheva, O., Iida, K.-i., Yoshida, S.-i. (2006). Temperature-Regulated Formation of Mycelial Mat-Like Biofilms by Legionella pneumophila. Appl. Environ. Microbiol. 72: 1613-1622 [Abstract] [Full Text]  
  • Bronstein, P. A., Marrichi, M., Cartinhour, S., Schneider, D. J., DeLisa, M. P. (2005). Identification of a Twin-Arginine Translocation System in Pseudomonas syringae pv. tomato DC3000 and Its Contribution to Pathogenicity and Fitness. J. Bacteriol. 187: 8450-8461 [Abstract] [Full Text]  
  • Dechesne, A., Pallud, C., Bertolla, F., Grundmann, G. L. (2005). Impact of the Microscale Distribution of a Pseudomonas Strain Introduced into Soil on Potential Contacts with Indigenous Bacteria. Appl. Environ. Microbiol. 71: 8123-8131 [Abstract] [Full Text]  
  • Kao, K. C., Tran, L. M., Liao, J. C. (2005). A Global Regulatory Role of Gluconeogenic Genes in Escherichia coli Revealed by Transcriptome Network Analysis. J. Biol. Chem. 280: 36079-36087 [Abstract] [Full Text]  
  • Karunakaran, R., Mauchline, T. H., Hosie, A. H. F., Poole, P. S. (2005). A family of promoter probe vectors incorporating autofluorescent and chromogenic reporter proteins for studying gene expression in Gram-negative bacteria. Microbiology 151: 3249-3256 [Abstract] [Full Text]  
  • Alper, H., Fischer, C., Nevoigt, E., Stephanopoulos, G. (2005). Tuning genetic control through promoter engineering. Proc. Natl. Acad. Sci. USA 102: 12678-12683 [Abstract] [Full Text]  
  • Norman, A., Hestbjerg Hansen, L., Sorensen, S. J. (2005). Construction of a ColD cda Promoter-Based SOS-Green Fluorescent Protein Whole-Cell Biosensor with Higher Sensitivity toward Genotoxic Compounds than Constructs Based on recA, umuDC, or sulA Promoters. Appl. Environ. Microbiol. 71: 2338-2346 [Abstract] [Full Text]  
  • Rasmussen, T. B., Bjarnsholt, T., Skindersoe, M. E., Hentzer, M., Kristoffersen, P., Kote, M., Nielsen, J., Eberl, L., Givskov, M. (2005). Screening for Quorum-Sensing Inhibitors (QSI) by Use of a Novel Genetic System, the QSI Selector. J. Bacteriol. 187: 1799-1814 [Abstract] [Full Text]  
  • Huber, B., Feldmann, F., Kothe, M., Vandamme, P., Wopperer, J., Riedel, K., Eberl, L. (2004). Identification of a Novel Virulence Factor in Burkholderia cenocepacia H111 Required for Efficient Slow Killing of Caenorhabditis elegans. Infect. Immun. 72: 7220-7230 [Abstract] [Full Text]  
  • Stapper, A. P., Narasimhan, G., Ohman, D. E., Barakat, J., Hentzer, M., Molin, S., Kharazmi, A., Hoiby, N., Mathee, K. (2004). Alginate production affects Pseudomonas aeruginosa biofilm development and architecture, but is not essential for biofilm formation. J Med Microbiol 53: 679-690 [Abstract] [Full Text]  
  • Basu, S., Mehreja, R., Thiberge, S., Chen, M.-T., Weiss, R. (2004). Spatiotemporal control of gene expression with pulse-generating networks. Proc. Natl. Acad. Sci. USA 101: 6355-6360 [Abstract] [Full Text]  
  • Bahl, M. I., Hansen, L. H., Licht, T. R., Sorensen, S. J. (2004). In Vivo Detection and Quantification of Tetracycline by Use of a Whole-Cell Biosensor in the Rat Intestine. Antimicrob. Agents Chemother. 48: 1112-1117 [Abstract] [Full Text]  
  • Bagge, N., Hentzer, M., Andersen, J. B., Ciofu, O., Givskov, M., Hoiby, N. (2004). Dynamics and Spatial Distribution of {beta}-Lactamase Expression in Pseudomonas aeruginosa Biofilms. Antimicrob. Agents Chemother. 48: 1168-1174 [Abstract] [Full Text]  
  • Boddicker, J. D., Jones, B. D. (2004). Lon Protease Activity Causes Down-Regulation of Salmonella Pathogenicity Island 1 Invasion Gene Expression after Infection of Epithelial Cells. Infect. Immun. 72: 2002-2013 [Abstract] [Full Text]  
  • Yarwood, J. M., Bartels, D. J., Volper, E. M., Greenberg, E. P. (2004). Quorum Sensing in Staphylococcus aureus Biofilms. J. Bacteriol. 186: 1838-1850 [Abstract] [Full Text]  
  • Bulter, T., Lee, S.-G., Wong, W. W., Fung, E., Connor, M. R., Liao, J. C. (2004). From the Cover: Design of artificial cell-cell communication using gene and metabolic networks. Proc. Natl. Acad. Sci. USA 101: 2299-2304 [Abstract] [Full Text]  
  • Labbate, M., Queck, S. Y., Koh, K. S., Rice, S. A., Givskov, M., Kjelleberg, S. (2004). Quorum Sensing-Controlled Biofilm Development in Serratia liquefaciens MG1. J. Bacteriol. 186: 692-698 [Abstract] [Full Text]  
  • Courville, P., Chaloupka, R., Veyrier, F., Cellier, M. F. M. (2004). Determination of Transmembrane Topology of the Escherichia coli Natural Resistance-associated Macrophage Protein (Nramp) Ortholog. J. Biol. Chem. 279: 3318-3326 [Abstract] [Full Text]  
  • Webb, J. S., Thompson, L. S., James, S., Charlton, T., Tolker-Nielsen, T., Koch, B., Givskov, M., Kjelleberg, S. (2003). Cell Death in Pseudomonas aeruginosa Biofilm Development. J. Bacteriol. 185: 4585-4592 [Abstract] [Full Text]  
  • Isaacs, F. J., Hasty, J., Cantor, C. R., Collins, J. J. (2003). Prediction and measurement of an autoregulatory genetic module. Proc. Natl. Acad. Sci. USA 100: 7714-7719 [Abstract] [Full Text]  
  • Axtell, C. A., Beattie, G. A. (2002). Construction and Characterization of a proU-gfp Transcriptional Fusion That Measures Water Availability in a Microbial Habitat. Appl. Environ. Microbiol. 68: 4604-4612 [Abstract] [Full Text]  
  • DeLisa, M. P., Samuelson, P., Palmer, T., Georgiou, G. (2002). Genetic Analysis of the Twin Arginine Translocator Secretion Pathway in Bacteria. J. Biol. Chem. 277: 29825-29831 [Abstract] [Full Text]  
  • Morgan-Kiss, R. M., Wadler, C., Cronan, J. E. Jr. (2002). Long-term and homogeneous regulation of the Escherichia coli araBAD promoter by use of a lactose transporter of relaxed specificity. Proc. Natl. Acad. Sci. USA 99: 7373-7377 [Abstract] [Full Text]  
  • Manefield, M., Rasmussen, T. B., Henzter, M., Andersen, J. B., Steinberg, P., Kjelleberg, S., Givskov, M. (2002). Halogenated furanones inhibit quorum sensing through accelerated LuxR turnover. Microbiology 148: 1119-1127 [Abstract] [Full Text]  
  • Hentzer, M., Riedel, K., Rasmussen, T. B., Heydorn, A., Andersen, J. B., Parsek, M. R., Rice, S. A., Eberl, L., Molin, S., Hoiby, N., Kjelleberg, S., Givskov, M. (2002). Inhibition of quorum sensing in Pseudomonas aeruginosa biofilm bacteria by a halogenated furanone compound. Microbiology 148: 87-102 [Abstract] [Full Text]  
  • Steidle, A., Sigl, K., Schuhegger, R., Ihring, A., Schmid, M., Gantner, S., Stoffels, M., Riedel, K., Givskov, M., Hartmann, A., Langebartels, C., Eberl, L. (2001). Visualization of N-Acylhomoserine Lactone-Mediated Cell-Cell Communication between Bacteria Colonizing the Tomato Rhizosphere. Appl. Environ. Microbiol. 67: 5761-5770 [Abstract] [Full Text]  
  • Leveau, J. H. J., Lindow, S. E. (2001). Predictive and Interpretive Simulation of Green Fluorescent Protein Expression in Reporter Bacteria. J. Bacteriol. 183: 6752-6762 [Abstract] [Full Text]  
  • Riedel, K., Hentzer, M., Geisenberger, O., Huber, B., Steidle, A., Wu, H., Hoiby, N., Givskov, M., Molin, S., Eberl, L. (2001). N-Acylhomoserine-lactone-mediated communication between Pseudomonas aeruginosa and Burkholderia cepacia in mixed biofilms. Microbiology 147: 3249-3262 [Abstract] [Full Text]  
  • Hentzer, M., Teitzel, G. M., Balzer, G. J., Heydorn, A., Molin, S., Givskov, M., Parsek, M. R. (2001). Alginate Overproduction Affects Pseudomonas aeruginosa Biofilm Structure and Function. J. Bacteriol. 183: 5395-5401 [Abstract] [Full Text]  
  • Flynn, J. M., Levchenko, I., Seidel, M., Wickner, S. H., Sauer, R. T., Baker, T. A. (2001). Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. USA 10.1073/pnas.191375298v1 [Abstract] [Full Text]  
  • Huber, B., Riedel, K., Hentzer, M., Heydorn, A., Gotschlich, A., Givskov, M., Molin, S., Eberl, L. (2001). The cep quorum-sensing system of Burkholderia cepacia H111 controls biofilm formation and swarming motility. Microbiology 147: 2517-2528 [Abstract] [Full Text]  
  • Lorang, J. M., Tuori, R. P., Martinez, J. P., Sawyer, T. L., Redman, R. S., Rollins, J. A., Wolpert, T. J., Johnson, K. B., Rodriguez, R. J., Dickman, M. B., Ciuffetti, L. M. (2001). Green Fluorescent Protein Is Lighting Up Fungal Biology. Appl. Environ. Microbiol. 67: 1987-1994 [Full Text]  
  • Hansen, M. C., Palmer, R. J. Jr, Udsen, C., White, D. C., Molin, S. (2001). Assessment of GFP fluorescence in cells of Streptococcus gordonii under conditions of low pH and low oxygen concentration. Microbiology 147: 1383-1391 [Abstract] [Full Text]  
  • De Kievit, T. R., Gillis, R., Marx, S., Brown, C., Iglewski, B. H. (2001). Quorum-Sensing Genes in Pseudomonas aeruginosa Biofilms: Their Role and Expression Patterns. Appl. Environ. Microbiol. 67: 1865-1873 [Abstract] [Full Text]  
  • Bringhurst, R. M., Cardon, Z. G., Gage, D. J. (2001). Galactosides in the rhizosphere: Utilization by Sinorhizobium meliloti and development of a biosensor. Proc. Natl. Acad. Sci. USA 10.1073/pnas.071375898v1 [Abstract] [Full Text]  
  • Leveau, J. H. J., Lindow, S. E. (2001). Inaugural Article: Appetite of an epiphyte: Quantitative monitoring of bacterial sugar consumption in the phyllosphere. Proc. Natl. Acad. Sci. USA 98: 3446-3453 [Abstract] [Full Text]  
  • Andersen, J. B., Heydorn, A., Hentzer, M., Eberl, L., Geisenberger, O., Christensen, B. B., Molin, S., Givskov, M. (2001). gfp-Based N-Acyl Homoserine-Lactone Sensor Systems for Detection of Bacterial Communication. Appl. Environ. Microbiol. 67: 575-585 [Abstract] [Full Text]  
  • Sarand, I., Skärfstad, E., Forsman, M., Romantschuk, M., Shingler, V. (2001). Role of the DmpR-Mediated Regulatory Circuit in Bacterial Biodegradation Properties in Methylphenol-Amended Soils. Appl. Environ. Microbiol. 67: 162-171 [Abstract] [Full Text]  
  • Rasmussen, T. B., Manefield, M., Andersen, J. B., Eberl, L., Anthoni, U., Christophersen, C., Steinberg, P., Kjelleberg, S., Givskov, M. (2000). How Delisea pulchra furanones affect quorum sensing and swarming motility in Serratia liquefaciens MG1. Microbiology 146: 3237-3244 [Abstract] [Full Text]  
  • Tolker-Nielsen, T., Brinch, U. C., Ragas, P. C., Andersen, J. B., Jacobsen, C. S., Molin, S. (2000). Development and Dynamics of Pseudomonas sp. Biofilms. J. Bacteriol. 182: 6482-6489 [Abstract] [Full Text]  
  • Heydorn, A., Nielsen, A. T., Hentzer, M., Sternberg, C., Givskov, M., Ersbøll, B. K., Molin, S. (2000). Quantification of biofilm structures by the novel computer program COMSTAT. Microbiology 146: 2395-2407 [Abstract] [Full Text]  
  • Heydorn, A., Ersbøll, B. K., Hentzer, M., Parsek, M. R., Givskov, M., Molin, S. (2000). Experimental reproducibility in flow-chamber biofilms. Microbiology 146: 2409-2415 [Abstract] [Full Text]  
  • Wu, H., Song, Z., Hentzer, M., Andersen, J. B., Heydorn, A., Mathee, K., Moser, C., Eberl, L., Molin, S., Høiby, N., Givskov, M. (2000). Detection of N-acylhomoserine lactones in lung tissues of mice infected with Pseudomonas aeruginosa. Microbiology 146: 2481-2493 [Abstract] [Full Text]  
  • Lowder, M., Unge, A., Maraha, N., Jansson, J. K., Swiggett, J., Oliver, J. D. (2000). Effect of Starvation and the Viable-but-Nonculturable State on Green Fluorescent Protein (GFP) Fluorescence in GFP-Tagged Pseudomonas fluorescens A506. Appl. Environ. Microbiol. 66: 3160-3165 [Abstract] [Full Text]  
  • Baty, A. M. III, Eastburn, C. C., Diwu, Z., Techkarnjanaruk, S., Goodman, A. E., Geesey, G. G. (2000). Differentiation of Chitinase-Active and Non-Chitinase-Active Subpopulations of a Marine Bacterium during Chitin Degradation. Appl. Environ. Microbiol. 66: 3566-3573 [Abstract] [Full Text]  
  • Whiteley, M., Parsek, M. R., Greenberg, E. P. (2000). Regulation of Quorum Sensing by RpoS in Pseudomonas aeruginosa. J. Bacteriol. 182: 4356-4360 [Abstract] [Full Text]  
  • Ramos, C., Mølbak, L., Molin, S. (2000). Bacterial Activity in the Rhizosphere Analyzed at the Single-Cell Level by Monitoring Ribosome Contents and Synthesis Rates. Appl. Environ. Microbiol. 66: 801-809 [Abstract] [Full Text]  
  • Cowan, S. E., Gilbert, E., Khlebnikov, A., Keasling, J. D. (2000). Dual Labeling with Green Fluorescent Proteins for Confocal Microscopy. Appl. Environ. Microbiol. 66: 413-418 [Abstract] [Full Text]  
  • Sternberg, C., Christensen, B. B., Johansen, T., Toftgaard Nielsen, A., Andersen, J. B., Givskov, M., Molin, S. (1999). Distribution of Bacterial Growth Activity in Flow-Chamber Biofilms. Appl. Environ. Microbiol. 65: 4108-4117 [Abstract] [Full Text]  
  • Christensen, B. B., Sternberg, C., Andersen, J. B., Eberl, L., Møller, S., Givskov, M., Molin, S. (1998). Establishment of New Genetic Traits in a Microbial Biofilm Community. Appl. Environ. Microbiol. 64: 2247-2255 [Abstract] [Full Text]  
  • Bringhurst, R. M., Cardon, Z. G., Gage, D. J. (2001). Galactosides in the rhizosphere: Utilization by Sinorhizobium meliloti and development of a biosensor. Proc. Natl. Acad. Sci. USA 98: 4540-4545 [Abstract] [Full Text]  
  • Flynn, J. M., Levchenko, I., Seidel, M., Wickner, S. H., Sauer, R. T., Baker, T. A. (2001). Overlapping recognition determinants within the ssrA degradation tag allow modulation of proteolysis. Proc. Natl. Acad. Sci. USA 98: 10584-10589 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»