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Appl Environ Microbiol, June 1998, p. 2278-2280, Vol. 64, No. 6
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Toxoplasma gondii Oocysts in Drinking Water

Judith Isaac-Renton,1,* William R. Bowie,2 Arlene King,3 G. Stewart Irwin,4 Corinne S. Ong,1 C. P. Fung,5 M. Omar Shokeir,1 and J. P. Dubey6

Department of Pathology and Laboratory Medicine1 and Division of Infectious Diseases, Department of Medicine,2 University of British Columbia, and Epidemiology Services3 and Provincial Laboratory,5 British Columbia Centre for Disease Control, British Columbia Ministry of Health, Vancouver, and Water Department, Capital Regional District,4 Victoria, British Columbia, Canada, and Parasite Biology and Epidemiology Laboratory, Livestock and Poultry Sciences Institute, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland6

Received 1 December 1997/Accepted 23 March 1998

The world's largest outbreak of waterborne toxoplasmosis occurred in a municipality in the western Canadian province of British Columbia. When drinking water emerged as a possible source of infection during the outbreak investigation, a laboratory method was needed to attempt detection of the parasite, Toxoplasma gondii. The method developed was based on the current U.S. Environmental Protection Agency method for detection of Cryptosporidium oocysts. Collection of large-volume drinking water samples and cartridge filter processing were unchanged, although identification of Toxoplasma oocysts in the filter retentate was carried out by using a previously described rodent model. Validation of the method developed was tested by using oocysts from a well-characterized Toxoplasma strain.

* Corresponding author. Mailing address: Division of Microbiology and Infection Control, Department of Pathology and Laboratory Medicine, Laurel Street Pavilion, Vancouver General Hospital, 855 West 12th, Vancouver, British Columbia, Canada V5Z 1M9. Phone: (604) 875-4631. Fax: (604) 875-4359. E-mail: isaacren{at}unixg.ubc.ca.

Appl Environ Microbiol, June 1998, p. 2278-2280, Vol. 64, No. 6
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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