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Appl Environ Microbiol, June 1998, p. 2284-2286, Vol. 64, No. 6
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Cyclospora cayetanensis in Wastewater

Gregory D. Sturbaum,1 Ynes R. Ortega,2 Robert H. Gilman,3,4,5,* Charles R. Sterling,2 Lilia Cabrera,5 and Donald A. Klein1

Department of Microbiology, Colorado State University, Ft. Collins, Colorado1; Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona2; Department of International Health, Johns Hopkins University, Baltimore, Maryland3; and Departamento de Patologia, Universidad Peruana Cayetano Heredia,4 and Asociacion Benefica PRISMA,5 Lima, Peru

Received 15 October 1997/Accepted 25 March 1998

Cyclospora cayetanensis causes diarrheal disease worldwide without a confirmed mode of transmission. Wastewater was examined for the presence of this organism. Oocysts were detected microscopically, and their identity was confirmed by molecular techniques. These findings verify that current techniques can isolate Cyclospora oocysts and suggest that fecally contaminated water may act as a vehicle of transmission.

* Corresponding author. Mailing address: Department of International Health, Johns Hopkins University, 615 N. Wolfe Street, Baltimore, MD 21205. Phone: (410) 614-3959. Fax: (410) 614-6060. E-mail: rgilman{at}jhsph.edu.

Appl Environ Microbiol, June 1998, p. 2284-2286, Vol. 64, No. 6
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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