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Appl Environ Microbiol, July 1998, p. 2418-2423, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Differentiation of Lactobacillus Species by Molecular Typing

Wei Zhong,1 Kevin Millsap,2,dagger Hanna Bialkowska-Hobrzanska,1,2 and Gregor Reid1,2,*

Lawson Research Institute1 and Department of Microbiology and Immunology, University of Western Ontario,2 London, Ontario, Canada

Received 27 October 1997/Accepted 12 April 1998

A total of 64 type, reference, clinical, health food, and stock isolates of microaerophilic Lactobacillus species were examined by restriction fragment length polymorphisms. Of particular interest were members of six of the eight species most commonly recovered from the vaginas of healthy premenopausal women, namely, Lactobacillus jensenii, L. casei, L. rhamnosus, L. acidophilus, L. plantarum, and L. fermentum. Six main groupings were identified on the basis of ribotyping. This technique was able to classify fresh isolates to the species level. In the case of the ribotype A grouping for L. rhamnosus, differences between strains were evident by chromosome typing (chromotyping). Many isolates did not possess plasmids. Six L. rhamnosus strains isolated from four different health food products appeared to be identical to L. rhamnosus ATCC 21052. The molecular typing system is useful for identifying and differentiating Lactobacillus isolates. Studies of strains of potential importance to the urogenital flora should include molecular characterization as a means of comparing genetic traits with those of strains whose characteristics associated with colonization and antagonism against pathogens have been defined.

* Corresponding author. Mailing address: H414, Lawson Research Institute, 268 Grosvenor St., London, Ontario N6A 4V2, Canada. Phone: 519-646-6100, ext. 5256. Fax: 519-646-6110. E-mail: gregor{at}julian.uwo.ca.

dagger Present address: Materia Technica, University of Groningen, Groningen, The Netherlands.

Appl Environ Microbiol, July 1998, p. 2418-2423, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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