This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Glynn, J. R.
Right arrow Articles by Baygents, J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Glynn, J. R., Jr.
Right arrow Articles by Baygents, J. C.
Agricola
Right arrow Articles by Glynn, J. R.
Right arrow Articles by Baygents, J. C.

 Previous Article  |  Next Article 

Appl Environ Microbiol, July 1998, p. 2572-2577, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Capillary Electrophoresis Measurements of Electrophoretic Mobility for Colloidal Particles of Biological Interest

J. R. Glynn Jr., B. M. Belongia, R. G. Arnold, K. L. Ogden, and J. C. Baygents*

Department of Chemical and Environmental Engineering, The University of Arizona, Tucson, Arizona 85721

Received 12 December 1997/Accepted 17 April 1998

The electrophoretic mobilities of three bacterial strains were investigated by capillary electrophoresis (CE) and were compared with results obtained by microelectrophoresis (ME). The CE measurements yielded bimodal electropherograms for two of the strains, thus illustrating for the first time that surface charge variations within a monoclonal population can be probed by CE. Intrapopulation variations were not detected by ME. The mobilities of three chemically distinct types of latex microspheres were also measured. Differences between the mean mobilities obtained by CE and ME were not statistically significant (P <=  0.50); the standard deviations of the CE measurements were typically 2 to 10 times smaller than those obtained by comparable ME measurements. The reproducibility of CE permitted batch-to-batch mobility variations to be probed for the bacteria (one of the strains exhibited such variations), and aggregation was evident in one of the latex suspensions. These effects were not measurable with ME.

* Corresponding author. Mailing address: Department of Chemical and Environmental Engineering, The University of Arizona, Tucson, AZ 85721. Phone: (520) 621-6043. Fax: (520) 621-6048. E-mail: jcb{at}maxwell.che.arizona.edu.

Appl Environ Microbiol, July 1998, p. 2572-2577, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • van Merode, A. E. J., van der Mei, H. C., Busscher, H. J., Krom, B. P. (2006). Influence of Culture Heterogeneity in Cell Surface Charge on Adhesion and Biofilm Formation by Enterococcus faecalis.. J. Bacteriol. 188: 2421-2426 [Abstract] [Full Text]  
  • van Merode, A. E. J., van der Mei, H. C., Busscher, H. J., Waar, K., Krom, B. P. (2006). Enterococcus faecalis strains show culture heterogeneity in cell surface charge.. Microbiology 152: 807-814 [Abstract] [Full Text]  
  • Butkus, M. A., Bays, J. T., Labare, M. P. (2003). Influence of Surface Characteristics on the Stability of Cryptosporidium parvum Oocysts. Appl. Environ. Microbiol. 69: 3819-3825 [Abstract] [Full Text]  
  • Desai, M. J., Armstrong, D. W. (2003). Separation, Identification, and Characterization of Microorganisms by Capillary Electrophoresis. Microbiol. Mol. Biol. Rev. 67: 38-51 [Abstract] [Full Text]  
  • Streger, S. H., Vainberg, S., Dong, H., Hatzinger, P. B. (2002). Enhancing Transport of Hydrogenophagaflava ENV735 for Bioaugmentation of Aquifers Contaminated with Methyl tert-Butyl Ether. Appl. Environ. Microbiol. 68: 5571-5579 [Abstract] [Full Text]  
  • Dong, H., Rothmel, R., Onstott, T. C., Fuller, M. E., DeFlaun, M. F., Streger, S. H., Dunlap, R., Fletcher, M. (2002). Simultaneous Transport of Two Bacterial Strains in Intact Cores from Oyster, Virginia: Biological Effects and Numerical Modeling. Appl. Environ. Microbiol. 68: 2120-2132 [Abstract] [Full Text]  
  • van der Mei, H. C., Busscher, H. J. (2001). Electrophoretic Mobility Distributions of Single-Strain Microbial Populations. Appl. Environ. Microbiol. 67: 491-494 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»