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Appl Environ Microbiol, July 1998, p. 2701-2704, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Enhanced Broth Media for Selective Growth of Vibrio vulnificusdagger

Wei-Yea Hsu,1,2 Cheng-i Wei,1 and Mark L. Tamplin1,2,*

Department of Food Science and Human Nutrition1 and Department of Family, Youth & Community Sciences,2 University of Florida, Gainesville, Florida 32611

Received 19 November 1997/Accepted 2 April 1998

Rapid detection of Vibrio vulnificus can be enhanced by optimizing the components of enrichment broth. PNC (5% peptone, 1% NaCl, and 0.08% cellobiose [pH 8.0]) enhanced the growth of V. vulnificus compared to alkaline peptone broth. PNCC (PNC with 1.0 to 4.1 U of colistin methanesulfonate per ml) increased the growth of low levels of V. vulnificus while suppressing non-target bacteria.

* Corresponding author. Mailing address: Department of Family, Youth & Community Sciences, Institute of Food and Agricultural Sciences, University of Florida, P.O. Box 110365, Gainesville, FL 32611-0365. Phone: (352) 392-2030. Fax: (352) 846-1102. E-mail: mlt{at}gnv.ifas.ufl.edu.

dagger Florida Agricultural Experiment Station Journal Series no. R-06330.

Appl Environ Microbiol, July 1998, p. 2701-2704, Vol. 64, No. 7
0099-2240/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Warner, E., Oliver, J. D. (2007). Refined Medium for Direct Isolation of Vibrio vulnificus from Oyster Tissue and Seawater. Appl. Environ. Microbiol. 73: 3098-3100 [Abstract] [Full Text]  
  • Cerdà-Cuéllar, M., Jofre, J., Blanch, A. R. (2000). A Selective Medium and a Specific Probe for Detection of Vibrio vulnificus. Appl. Environ. Microbiol. 66: 855-859 [Abstract] [Full Text]  


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